Structure of lysophosphatidylglyceryl-Remazolbrilliant Blue (LPG-RBB) from the surface of dyed wheat starch granules

被引:3
作者
Seguchi, M [1 ]
Kanenaga, K [1 ]
机构
[1] Kobe Womens Univ, Lab Food Technol, Fac Home Technol, Suma Ku, Kobe City 614, Japan
关键词
D O I
10.1094/CCHEM.1998.75.3.294
中图分类号
O69 [应用化学];
学科分类号
081704 ;
摘要
Wheat starch granule surface was covalently stained with Remazol-brilliant blue-R dye (RBB) and then extracted with 1% SDS containing 1% 2-mercaptoethanol (2-ME) at room temperature for 14.5 hr. The extracted blue-staining material (A(650)) separated into two fractions. Low molecular weight (LMW) material was further purified by Sephadex G-75 size-exclusion chromatography and thin-layer chromatography. infrared and nuclear magnetic resonance (H-1-NMR and C-13-NMR) spectroscopy indicated that the structure of the purified LMW material was 18-O-(6-lysophosphatidylglyceryl)-RBB.
引用
收藏
页码:294 / 296
页数:3
相关论文
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