Phosphatidate phosphatases and diacylglycerol pyrophosphate phosphatases in Saccharomyces cerevisiae and Escherichia coli

被引:67
作者
Carman, GM
机构
[1] Department of Food Science, Cook College, New Jersey Agric. Exp. Station, New Brunswick
来源
BIOCHIMICA ET BIOPHYSICA ACTA-LIPIDS AND LIPID METABOLISM | 1997年 / 1348卷 / 1-2期
关键词
phosphatidate; diacylglycerol; diacylglycerol pyrophosphate; phosphatases; yeast; bacteria;
D O I
10.1016/S0005-2760(97)00095-7
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Phosphatidate phosphatase plays a major role in the synthesis of phospholipids and triacylglycerols in the yeast Saccharomyces cerevisiae. Membrane- and cytosolic-associated forms of the enzyme have been isolated and characterized. These enzymes are Mg2+-dependent and N-ethylmaleimide-sensitive. The expression of a membrane-associated form of phosphatidate phosphatase is regulated by growth phase and inositol supplementation, whereas enzyme activity is regulated by lipids, nucleotides, and by phosphorylation. Phosphatidate phosphatase is coordinately regulated with other phospholipid biosynthetic enzymes including phosphatidylserine synthase. Diacylglycerol pyrophosphate phosphatase is a novel enzyme of phospholipid metabolism which is present in S. cerevisiae, Escherichia coli, and mammalian cells. This enzyme possesses a phosphatidate phosphatase activity which is Mg2+-independent and N-ethylmaleimide-insensitive and is distinct from the Mg2+-dependent and N-ethylmaleimide-sensitive form of phosphatidate phosphatase. Genes encoding for diacylglycerol pyrophosphate phosphatase have been isolated from S. cerevisiae and E. coli. The deduced protein sequences of these genes show homology to the sequence of the mouse PAP2 (Mg2+-independent and N-ethylmaleimide-insensitive phosphatidate phosphatase) protein, especially in a novel phosphatase sequence motif. Rat liver PAP2 displays diacylglycerol pyrophosphate phosphatase activity. (C) 1997 Elsevier Science B.V.
引用
收藏
页码:45 / 55
页数:11
相关论文
共 58 条
  • [11] Dillon DA, 1997, J BIOL CHEM, V272, P10361
  • [12] The Escherichia coli pgpB gene encodes for a diacylglycerol pyrophosphate phosphatase activity
    Dillon, DA
    Wu, WI
    Riedel, B
    Wissing, JB
    Dowhan, W
    Carman, GM
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 1996, 271 (48) : 30548 - 30553
  • [13] PHOSPHOLIPID SIGNALING
    DIVECHA, N
    IRVINE, RF
    [J]. CELL, 1995, 80 (02) : 269 - 278
  • [14] MYOINOSITOL METABOLITES AS CELLULAR SIGNALS
    DOWNES, CP
    MACPHEE, CH
    [J]. EUROPEAN JOURNAL OF BIOCHEMISTRY, 1990, 193 (01): : 1 - 18
  • [15] Messenger functions of phosphatidic acid
    English, D
    Cui, Y
    Siddiqui, RA
    [J]. CHEMISTRY AND PHYSICS OF LIPIDS, 1996, 80 (1-2) : 117 - 132
  • [16] Phosphatidic acid: A lipid messenger involved in intracellular and extracellular signalling
    English, D
    [J]. CELLULAR SIGNALLING, 1996, 8 (05) : 341 - 347
  • [17] PHOSPHATIDYLCHOLINE BREAKDOWN AND SIGNAL-TRANSDUCTION
    EXTON, JH
    [J]. BIOCHIMICA ET BIOPHYSICA ACTA-LIPIDS AND LIPID METABOLISM, 1994, 1212 (01): : 26 - 42
  • [18] SRV2, A GENE REQUIRED FOR RAS ACTIVATION OF ADENYLATE-CYCLASE IN YEAST
    FEDORCHAIKEN, M
    DESCHENES, RJ
    BROACH, JR
    [J]. CELL, 1990, 61 (02) : 329 - 340
  • [19] PURIFICATION AND CHARACTERIZATION OF N-ETHYLMALEIMIDE-INSENSITIVE PHOSPHATIDIC-ACID PHOSPHOHYDROLASE (PAP2) FROM RAT-LIVER
    FLEMING, IN
    YEAMAN, SJ
    [J]. BIOCHEMICAL JOURNAL, 1995, 308 : 983 - 989
  • [20] THE PGPA AND PGPB GENES OF ESCHERICHIA-COLI ARE NOT ESSENTIAL - EVIDENCE FOR A 3RD PHOSPHATIDYLGLYCEROPHOSPHATE PHOSPHATASE
    FUNK, CR
    ZIMNIAK, L
    DOWHAN, W
    [J]. JOURNAL OF BACTERIOLOGY, 1992, 174 (01) : 205 - 213