A test for adequacy of chromium nutrition in humans-relation to Type 2 diabetes mellitus

When (Na2CrO4)-Cr-51 is added to blood the (CrO42-)-Cr-51 ions enter the erythrocytes readily, and nearly exclusively, and are reduced to Cr-51(III) ions. We have observed that a fraction of these ions becomes bound to the cell membrane in a concentration which seemingly depends on that of the dietary derived intracellular Cr(Ill) ions. Thus, when constant amounts of (51)Cro(4)(2-) ions enter constant amounts of erythrocytes, the resulting Cr-51(III) ions become bound to the cell membrane in a concentration that varies inversely as the initial, intracellular concentration of Cr(III) ions which, in turn, depends directly on the adequacy of chromium nutrition. Therefore, we have determined an arbitrary set of conditions under which the concentration of Cr-51(Ill) ions bound to the erythrocyte membrane becomes an indicator of the adequacy of chromium nutrition. The application of this test to 25 Type 2 diabetes mellitus subjects and 35 normal controls, both randomly selected, indicates that the concentrations of membrane bound Cr-51(111) ions in the two groups were not significantly different. Consequently, it is concluded that the level of chromium nutrition which is normally adequate in humans has only a minor role, if any, in the genesis of Type 2 diabetes mellitus. (C) 2003 Elsevier Science (USA). All rights reserved.