Feasibility of supercritical fluid chromatography chemiluminescent nitrogen detection with open tubular columns

被引：15

作者：

Shi, H

Strode, JTB

Taylor, LT

Fujinari, EM

机构：

[1] VIRGINIA POLYTECH INST & STATE UNIV, DEPT CHEM, BLACKSBURG, VA 24060 USA

[2] ANTEK INSTRUMENTS INC, HOUSTON, TX 77090 USA

来源：

JOURNAL OF CHROMATOGRAPHY A | 1996年 / 734卷 / 02期

关键词：

supercritical fluid chromatography; detectors; SFC; chemiluminescence detector; indoles; nitrotoluenes; pyridine; caffeine; diphenylamine; thiocyanates;

D O I：

10.1016/0021-9673(95)01191-9

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A supercritical fluid chromatography-chemiluminescence nitrogen detection (SFC-CLND) system for open tubular columns is described. The chemistry for the post-restrictor detector is based on the chemiluminescent reaction between ozone generated from oxygen and nitric oxide via high-temperature pyrolysis of nitrogen-containing compounds. The minimum detectable quantity is found to be 60 pg nitrogen of indole by flow injection analysis. Detector linearity was at least three orders of magnitude, and a selectivity of 10(5) was obtained. Several applications of SFC-CLND in the analysis of compounds containing nitrogen are presented.

引用

页码：303 / 310

页数：8

共 18 条

[1]

BENN SM, 1993, DEV FOOD SCI, V32, P65

[2]

BURNHOP DJ, 1989, ANAL CHEM, V61, P797

[3] USE OF SULFUR CHEMILUMINESCENCE DETECTION AFTER SUPERCRITICAL FLUID CHROMATOGRAPHY [J].