Carotenoid biosynthesis during tomato fruit development:: regulatory role of 1-deoxy-D-xylulose 5-phosphate synthase

被引:331
作者
Lois, LM [1 ]
Rodríguez-Concepción, M [1 ]
Gallego, F [1 ]
Campos, N [1 ]
Boronat, A [1 ]
机构
[1] Univ Barcelona, Fac Quim, Dept Bioquim & Biol Mol, E-08028 Barcelona, Spain
关键词
D O I
10.1046/j.1365-313x.2000.00764.x
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Plant isoprenoids represent a heterogeneous group of compounds which play essential roles not only in growth and development, but also in the interaction of plants with their environment. Higher plants contain two pathways for the biosynthesis of isoprenoids: the mevalonate pathway, located in the cytosol/endoplasmic reticulum, and the recently discovered mevalonate-independent pathway (Rohmer pathway), located in the plastids. In order to evaluate the function of the Rohmer pathway in the regulation of the synthesis of plastidial isoprenoids, we have isolated a tomato cDNA encoding 1-deoxy-D-xylulose 5-phosphate synthase (DXS), the first enzyme of the pathway. We demonstrate in vivo activity and plastid targeting of plant DXS. Expression analysis of the tomato DXS gene indicates developmental and organ-specific regulation of mRNA accumulation and a strong correlation with carotenoid synthesis during fruit development. 1-Deoxy-D-xylulose feeding experiments, together with expression analysis of DXS and PSY1 (encoding the fruit-specific isoform of phytoene synthase) in wild-type and yellow flesh mutant fruits, indicate that DXS catalyses the first potentially regulatory step in carotenoid biosynthesis during early fruit ripening. Our results change the current view that PSY1 is the only regulatory enzyme in tomato fruit carotenogenesis, and point towards a coordinated role of both DXS and PSY1 in the control of fruit carotenoid synthesis.
引用
收藏
页码:503 / 513
页数:11
相关论文
共 36 条
[21]   The 1-deoxy-D-xylulose-5-phosphate pathway of isoprenoid biosynthesis in plants [J].
Lichtenthaler, HK .
ANNUAL REVIEW OF PLANT PHYSIOLOGY AND PLANT MOLECULAR BIOLOGY, 1999, 50 :47-65
[22]   Cloning and characterization of a gene from Escherichia coli encoding a transketolase-like enzyme that catalyzes the synthesis of D-1-deoxyxylulose 5-phosphate, a common precursor for isoprenoid, thiamin, and pyridoxol biosynthesis [J].
Lois, LM ;
Campos, N ;
Putra, SR ;
Danielsen, K ;
Rohmer, M ;
Boronat, A .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (05) :2105-2110
[23]   CLA1, a novel gene required for chloroplast development, is highly conserved in evolution [J].
Mandel, MA ;
Feldmann, KA ;
HerreraEstrella, L ;
RochaSosa, M ;
Leon, P .
PLANT JOURNAL, 1996, 9 (05) :649-658
[24]   TERPENOID METABOLISM [J].
MCGARVEY, DJ ;
CROTEAU, R .
PLANT CELL, 1995, 7 (07) :1015-1026
[25]   The prenylation status of a novel plant calmodulin directs plasma membrane or nuclear localization of the protein [J].
Rodríguez-Concepción, M ;
Yalovsky, S ;
Zik, M ;
Fromm, H ;
Gruissem, W .
EMBO JOURNAL, 1999, 18 (07) :1996-2007
[26]   Arachidonic acid alters tomato HMG expression and fruit growth and induces 3-hydroxy-3-methylglutaryl coenzyme A reductase-independent lycopene accumulation [J].
Rodríguez-Concepción, M ;
Gruissem, W .
PLANT PHYSIOLOGY, 1999, 119 (01) :41-48
[27]   The discovery of a mevalonate-independent pathway for isoprenoid biosynthesis in bacteria, algae and higher plants [J].
Rohmer, M .
NATURAL PRODUCT REPORTS, 1999, 16 (05) :565-574
[28]   Glyceraldehyde 3-phosphate and pyruvate as precursors of isoprenic units in an alternative non-mevalonate pathway for terpenoid biosynthesis [J].
Rohmer, M ;
Seemann, M ;
Horbach, S ;
BringerMeyer, S ;
Sahm, H .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1996, 118 (11) :2564-2566
[29]   ISOPRENOID BIOSYNTHESIS IN BACTERIA - A NOVEL PATHWAY FOR THE EARLY STEPS LEADING TO ISOPENTENYL DIPHOSPHATE [J].
ROHMER, M ;
KNANI, M ;
SIMONIN, P ;
SUTTER, B ;
SAHM, H .
BIOCHEMICAL JOURNAL, 1993, 295 :517-524
[30]   Regulation of carotenoid biosynthesis during tomato fruit development:: Expression of the gene for lycopene epsilon-cyclase is down-regulated during ripening and is elevated in the mutant Delta [J].
Ronen, G ;
Cohen, M ;
Zamir, D ;
Hirschberg, J .
PLANT JOURNAL, 1999, 17 (04) :341-351