Intracellular osmotic action

被引：42

作者：

Rand, RP ^{[1
]}

Parsegian, VA

Rau, DC

机构：

[1] Brock Univ, Dept Biol Sci, St Catharines, ON L2S 3A1, Canada

[2] NICHHD, Lab Phys & Struct Biol, NIH, Bethesda, MD 20892 USA

来源：

CELLULAR AND MOLECULAR LIFE SCIENCES | 2000年 / 57卷 / 07期

关键词：

water; osmotic stress; channels; enzymes; DNA; actin;

D O I：

10.1007/PL00000742

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Water often acts as a critical reactant in cellular reactions. Its role can be detected by modulating water activity with osmotic agents. We describe the principles behind this 'osmotic stress' strategy, and survey the ubiquity of water effects on molecular structures that have aqueous, solute-excluding regions. These effects are seen with single-functioning molecules such as membrane channels and solution enzymes, as well as in the molecular assembly of actin, the organization of DNA and the specificity of protein/DNA interactions.

引用

页码：1018 / 1032

页数：15

共 41 条

[1] PROBING ALAMETHICIN CHANNELS WITH WATER-SOLUBLE POLYMERS - EFFECT ON CONDUCTANCE OF CHANNEL STATES [J].

BEZRUKOV, SM ;

VODYANOY, I .

BIOPHYSICAL JOURNAL, 1993, 64 (01) :16-25

[2] PROTEIN SOLVATION IN ALLOSTERIC REGULATION - A WATER EFFECT ON HEMOGLOBIN [J].

COLOMBO, MF ;

RAU, DC ;

PARSEGIAN, VA .

SCIENCE, 1992, 256 (5057) :655-659

[3] HYPERSENSITIVITY OF AN ENZYME REACTION TO SOLVENT WATER [J].

DZINGELESKI, GD ;

WOLFENDEN, R .

BIOCHEMISTRY, 1993, 32 (35) :9143-9147

[4] Water in actin polymerization [J].

Fuller, N ;

Rand, RP .

BIOPHYSICAL JOURNAL, 1999, 76 (06) :3261-3266

[5] WATER RELEASE ASSOCIATED WITH SPECIFIC BINDING OF GAL REPRESSOR [J].

GARNER, MM ;

RAU, DC .

EMBO JOURNAL, 1995, 14 (06) :1257-1263

[6] Role of water in protein kinase C catalysis and its binding to membranes [J].

Giorgione, JR ;

Epand, RM .

BIOCHEMISTRY, 1997, 36 (08) :2250-2256

[7] RESPONSES OF 2 PROTEIN-PROTEIN COMPLEXES TO SOLVENT STRESS - DOES WATER PLAY A ROLE AT THE INTERFACE [J].

KORNBLATT, JA ;

KORNBLATT, MJ ;

HOA, GHB ;

MAUK, AG .

BIOPHYSICAL JOURNAL, 1993, 65 (03) :1059-1065

[8] A NONTRADITIONAL ROLE FOR WATER IN THE CYTOCHROME-C-OXIDASE REACTION [J].

KORNBLATT, JA ;

HOA, GHB .

BIOCHEMISTRY, 1990, 29 (40) :9370-9376

[9] HYDRATION FORCES [J].

LEIKIN, S ;

PARSEGIAN, VA ;

RAU, DC ;

RAND, RP .

ANNUAL REVIEW OF PHYSICAL CHEMISTRY, 1993, 44 (01) :369-395

[10] DIRECT MEASUREMENT OF FORCES BETWEEN SELF-ASSEMBLED PROTEINS - TEMPERATURE-DEPENDENT EXPONENTIAL FORCES BETWEEN COLLAGEN TRIPLE HELICES [J].

LEIKIN, S ;

RAU, DC ;

PARSEGIAN, VA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (01) :276-280

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