Modeling the evolution of culture-adapted human embryonic stem cells

被引：48

作者：

Olariu, Victor ^{[2
]}

Harrison, Neil J. ^{[1
]}

Coca, Daniel ^{[2
]}

Gokhale, Paul J. ^{[1
]}

Baker, Duncan ^{[3
]}

Billings, Steve ^{[2
]}

Kadirkamanathan, Visakan ^{[2
]}

Andrews, Peter W. ^{[1
]}

机构：

[1] Univ Sheffield, Dept Biomed Sci, Sheffield S10 2TN, S Yorkshire, England

[2] Univ Sheffield, Dept Automat Control & Syst Engn, Sheffield S1 3JD, S Yorkshire, England

[3] Sheffield Childrens Trust, N Trent Clin Cytogenet Serv, Sheffield S10 2TH, S Yorkshire, England

来源：

STEM CELL RESEARCH | 2010年 / 4卷 / 01期

基金：

英国工程与自然科学研究理事会; 英国生物技术与生命科学研究理事会;

关键词：

CHROMOSOME; MUTATION; POPULATIONS;

D O I：

10.1016/j.scr.2009.09.001

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

The long-term culture of human embryonic stem (ES) cells is inevitably subject to evolution, since any mutant that arises with a growth advantage wilt be selectively amplified. However, the evolutionary influences of population size, mutation rate, and selection pressure are frequently overlooked. We have constructed a Monte Carlo simulation model to predict how changes in these factors can influence the appearance and spread of mutant ES cells, and verified its applicability by comparison with in vitro data. This simulation provides an estimate for the expected rate of generation of culture-adapted ES cells under different assumptions for the key parameters. In particular, it highlights the effect of population size, suggesting that the maintenance of cells in small populations reduces the likelihood that abnormal cultures wilt develop. (C) 2009 Elsevier B.V. All rights reserved.

引用

页码：50 / 56

页数：7

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