Heparin-Mimicking Multilayer Coating on Polymeric Membrane via LbL Assembly of Cyclodextrin-Based Supramolecules

被引：82

作者：

Deng, Jie ^{[1
]}

Liu, Xinyue ^{[1
]}

Ma, Lang ^{[1
]}

Cheng, Chong ^{[1
,2
]}

Shi, Wenbin ^{[1
]}

Nie, Chuanxiong ^{[1
]}

Zhao, Changsheng ^{[1
,3
]}

机构：

[1] Sichuan Univ, State Key Lab Polymer Mat Engn, Coll Polymer Sci & Engn, Chengdu 610065, Peoples R China

[2] Univ Michigan, Dept Biomed Engn, Dept Chem Engn, Ann Arbor, MI 48109 USA

[3] Sichuan Univ, Natl Engn Res Ctr Biomat, Chengdu 610064, Peoples R China

来源：

ACS APPLIED MATERIALS & INTERFACES | 2014年 / 6卷 / 23期

基金：

中国国家自然科学基金;

关键词：

beta-cyclodextrin; star-shaped supramolecules; layer-by-layer assembly; heparin-mimicking multilayer; blood and cell compatibility; polymeric membranes; SURFACE MODIFICATION; BLOOD COMPATIBILITY; CELL-PROLIFERATION; ENDOTHELIAL-CELLS; CATIONIC POLYMERS; MACRO-INTERFACE; GENE DELIVERY; IN-VITRO; LAYER; BIOCOMPATIBILITY;

D O I：

10.1021/am506249r

中图分类号：

TB3 [工程材料学];

学科分类号：

082905 [生物质能源与材料];

摘要：

In this study, multifunctional and heparin-mimicking star-shaped supramolecules-deposited 3D porous multilayer films with improved biocompatibility were fabricated via a layer-by-layer (LbL) self-assembly method on polymeric membrane substrates. Star-shaped heparin-mimicking polyanions (including poly(styrenesulfonate-co-sodium acrylate; Star-PSS-AANa) and poly(styrenesulfonate-co-poly(ethylene glycol)methyl ether methacrylate; Star-PSS-EGMA)) and polycations (poly(methyl chloride-quaternized 2-(dimethylamino)ethyl methacrylate; Star-PMeDMA) were first synthesized by atom transfer radical polymerization (ATRP) from beta-cyclodextrin (beta-CD) based cores. Then assembly of 3D porous multilayers onto polymeric membrane surfaces was carried out by alternating deposition of the polyanions and polycations via electrostatic interaction. The surface morphology and composition, water contact angle, blood activation, and thrombotic potential as well as cell viability for the coated heparin-mimicking films were systematically investigated. The results of surface ATR-FTIR spectra and XPS spectra verified successful deposition of the star-shaped supramolecules onto the biomedical membrane surfaces; scanning electron microscopy (SEM) and atomic force microscopy (AFM) observations revealed that the modified substrate had 3D porous surface morphology, which might have a great biological influence on the biointerface. Furthermore, systematic in vitro investigation of protein adsorption, platelet adhesion, human platelet factor 4 (PF4, indicates platelet activation), activate partial thromboplastin time (APTT), thrombin time (TT), coagulation activation (thrombin-antithrombin III complex (TAT, indicates blood coagulant)), and blood-related complement activation (C3a and C5a, indicates inflammation potential) confirmed that the heparin-mimicking multilayer coated membranes exhibited ultralow blood component activations and excellent hemocompatibility. Meanwhile, after surface coating, endothelial cell viability was also promoted, which indicated that the heparin-mimicking multilayer coating might extend the application fields of polymeric membranes in biomedical fields.

引用

页码：21603 / 21614

页数：12

共 49 条

[1]

Layer-by-layer self-assembled shells for drug delivery [J].