Crystallization and preliminary X-ray diffraction studies of a mosquito-larvicidal toxin from Bacillus thuringiensis subsp israelensis

被引：9

作者：

Boonserm, P

Ellar, DJ

Li, JD

机构：

[1] Univ Cambridge, Dept Biochem, Cambridge CB2 1GA, England

[2] MRC, Mol Biol Lab, Cambridge CB2 2QH, England

来源：

ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY | 2003年 / 59卷

关键词：

D O I：

10.1107/S0907444903001513

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The Cry4B delta-endotoxin from Bacillus thuringiensis subsp. israelensis is specifically toxic to mosquito larvae. For a better understanding of the mechanism of toxicity, chymotrypsin-activated Cry4B toxin (68 kDa) has been purified and crystallized in sodium bromide at neutral pH. The well formed crystals belong to the rhombohedral space group R32, with unit-cell parameters a = b = 185.82, c = 187.93 Angstrom, and diffracted X-rays to 1.75 Angstrom resolution. The asymmetric unit contains one toxin molecule and 74% solvent content, as shown by molecular replacement from a composite model of the homologous Cry3A and Cry1Aa. The purified protein and crystals both possessed mosquitocidal activity.

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页码：591 / 594

页数：4

相关论文

共 32 条

[1]

AHMAD W, 1990, FEMS MICROBIOL LETT, V68, P97, DOI 10.1111/j.1574-6968.1990.tb04130.x

[2]

ANGSUTHANASOMBAT C, 1991, FEMS MICROBIOL LETT, V83, P273, DOI 10.1111/j.1574-6968.1991.tb04476.x

[3] THE CCP4 SUITE - PROGRAMS FOR PROTEIN CRYSTALLOGRAPHY [J].

BAILEY, S .

ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, 1994, 50 :760-763

[4] N-acetylgalactosamine on the putative insect receptor aminopeptidase N is recognised by a site on the domain III lectin-like fold of a Bacillus thuringiensis insecticidal toxin [J].

Burton, SL ;

Ellar, DJ ;

Li, J ;

Derbyshire, DJ .

JOURNAL OF MOLECULAR BIOLOGY, 1999, 287 (05) :1011-1022

[5] SITE-DIRECTED MUTATIONS IN A HIGHLY CONSERVED REGION OF BACILLUS-THURINGIENSIS DELTA-ENDOTOXIN AFFECT INHIBITION OF SHORT-CIRCUIT CURRENT ACROSS BOMBYX-MORI MIDGUTS [J].

CHEN, XJ ;

LEE, MK ;

DEAN, DH .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (19) :9041-9045

[6] Revision of the nomenclature for the Bacillus thuringiensis pesticidal crystal proteins [J].

Crickmore, N ;

Zeigler, DR ;

Feitelson, J ;

Schnepf, E ;

Van Rie, J ;

Lereclus, D ;

Baum, J ;

Dean, DH .

MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS, 1998, 62 (03) :807-+

[7] Different domains of Bacillus thuringiensis delta-endotoxins can bind to insect midgut membrane proteins on ligand blots [J].

DeMaagd, RA ;

VanderKlei, H ;

Bakker, PL ;

Stiekema, WJ ;

Bosch, D .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1996, 62 (08) :2753-2757

[8] Crystallization of the Bacillus thuringiensis toxin Cry1Ac and its complex with the receptor ligand N-acetyl-D-galactosamine [J].

Derbyshire, DJ ;

Ellar, DJ ;

Li, J .

ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY, 2001, 57 :1938-1944

[9] Structure of the insecticidal bacterial δ-endotoxin Cry3Bb1 of Bacillus thuringiensis [J].

Galitsky, N ;

Cody, V ;

Wojtczak, A ;

Ghosh, D ;

Luft, JR ;

Pangborn, W ;

English, L .

ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY, 2001, 57 :1101-1109

[10] LOCATION OF THE BOMBYX-MORI SPECIFICITY DOMAIN ON A BACILLUS-THURINGIENSIS DELTA-ENDOTOXIN PROTEIN [J].

GE, AZ ;

SHIVAROVA, NI ;

DEAN, DH .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (11) :4037-4041

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