Crystallization and preliminary X-ray diffraction studies of a mosquito-larvicidal toxin from Bacillus thuringiensis subsp israelensis

被引：9

作者：

Boonserm, P

Ellar, DJ

Li, JD

机构：

[1] Univ Cambridge, Dept Biochem, Cambridge CB2 1GA, England

[2] MRC, Mol Biol Lab, Cambridge CB2 2QH, England

来源：

ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY | 2003年 / 59卷

关键词：

D O I：

10.1107/S0907444903001513

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The Cry4B delta-endotoxin from Bacillus thuringiensis subsp. israelensis is specifically toxic to mosquito larvae. For a better understanding of the mechanism of toxicity, chymotrypsin-activated Cry4B toxin (68 kDa) has been purified and crystallized in sodium bromide at neutral pH. The well formed crystals belong to the rhombohedral space group R32, with unit-cell parameters a = b = 185.82, c = 187.93 Angstrom, and diffracted X-rays to 1.75 Angstrom resolution. The asymmetric unit contains one toxin molecule and 74% solvent content, as shown by molecular replacement from a composite model of the homologous Cry3A and Cry1Aa. The purified protein and crystals both possessed mosquitocidal activity.

引用

页码：591 / 594

页数：4

共 32 条

[11] BACILLUS-THURINGIENSIS CRYLA(A) INSECTICIDAL TOXIN - CRYSTAL-STRUCTURE AND CHANNEL FORMATION [J].