Identification of morphine in the adrenal medullary chromaffin PC-12 cell line

被引：27

作者：

Goumon, Y

Zhu, W

Weeks, BS

Casares, F

Cadet, P

Bougaeva, M

Brownawell, B

Stefano, GB ^{[1
]}

机构：

[1] SUNY Coll Old Westbury, Neurosci Res Inst, Old Westbury, NY 11568 USA

[2] Adelphi Univ, Dept Biol, Garden City, NY 11530 USA

[3] SUNY Stony Brook, Marine Sci Res Ctr, Stony Brook, NY 11794 USA

来源：

MOLECULAR BRAIN RESEARCH | 2000年 / 81卷 / 1-2期

关键词：

rat; endogenous morphine; adrenal gland; nitric oxide;

D O I：

10.1016/S0169-328X(00)00141-8

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Morphine was identified in the adrenal medulla chromaffin PC-12 cell lime by reversed-phase HPLC, following liquid and solid extraction. The morphine corresponding HPLC fractions (1.746+/-0.615 ng of morphine/million cells) were further analyzed by gas chromatography-mass spectrometry and found to be identical to synthetic morphine. Furthermore, using primers derived from the human neuronal mu 1 opiate receptor, we used RT-PCR to detect expression of mu transcripts from this cell line. The transcript was absent. The study conclusively proves morphine, but not a mu 1 opiate receptor, is constitutively expressed in the adrenal medulla chromaffin PC-12 cell line. (C) 2000 Elsevier Science BN. All rights reserved.

引用

页码：177 / 180

页数：4

共 17 条

[1] Comparison of solid-phase extraction and supercritical fluid extraction for the analysis of morphine in whole blood [J].