Fast screening method for the profile analysis of polycyclic aromatic hydrocarbon metabolites in urine using derivatisation - solid-phase microextraction

被引：79

作者：

Gmeiner, G ^{[1
]}

Krassnig, C

Schmid, E

Tausch, H

机构：

[1] Austrian Res Ctr Siebersdorf, Dept Environm Anal, A-2444 Seibersdorf, Austria

[2] Univ Vienna, Inst Analyt Chem, A-1090 Vienna, Austria

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 1998年 / 705卷 / 01期

关键词：

polynuclear aromatic hydrocarbons; hydroxypyrene; hydroxyphenanthrene; hydroxynaphthalene;

D O I：

10.1016/S0378-4347(97)00526-4

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A method for the qualitative analysis of various metabolites of naphthalane, phenanthrene and pyrene is presented. The method uses SPME with a 85-mu m polyacrylate fibre for extraction, headspace silylation with BSTFA without any catalyst for on-fibre derivatisation and GC-MS in the SIR mode for separation and detection. The suitability of the method for profile analysis of PAH metabolites is shown by analysing a smokers urine after enzymatic cleavage (and additionally after spiking with the target analytes) and spiked water. The method exhibits satisfactory separation of all investigated metabolites and no interferences due to matrix peaks. (C) 1998 Elsevier Science B.V.

引用

页码：132 / 138

页数：7

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