Rho kinase is required for CCR7-mediated polarization and chemotaxis of T lymphocytes

被引：57

作者：

Bardi, G

Niggli, V

Loetscher, P

机构：

[1] Univ Bern, Theodor Kocher Inst, CH-3000 Bern, Switzerland

[2] Univ Bern, Dept Pathol, CH-3010 Bern, Switzerland

来源：

FEBS LETTERS | 2003年 / 542卷 / 1-3期

关键词：

rho kinase; extracellular signal-regulated kinase-2; polarization; chemotaxis; chemokine; chemokine receptor;

D O I：

10.1016/S0014-5793(03)00351-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

We studied the role of Rho kinase and extracellular signal-regulated kinase (ERK)-2 in the polarization and migration of T lymphocytes in response to the CCR7 ligands EBI1 ligand chemokine (ELC; CCL19) and secondary lymphoid-tissue chemokine (SLC; CCL21). Both Rho kinase protein isoforms are expressed in T lymphocytes. Inhibition of the Rho kinases with Y-27632 strongly inhibited SLC- and ELC-induced polarized morphology and chemotaxis of T lymphocytes. Although the chemokines induced ERK-2 activation, the blockade of this signaling pathway showed no effect on polarization and migration. This study indicates an important role of Rho kinase in CCR7-mediated polarization and migration of T lymphocytes, whereas ERK-2 is not involved in these processes. (C) 2003 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：79 / 83

页数：5

共 26 条

[1]

The functional role of Rho and Rho-associated coiled-coil forming protein kinase in eotaxin signaling of eosinophils [J].