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Detection and modelling of DNA hybridization by EIS measurements -: Mention of a polythiophene matrix suitable for electrochemically controlled gene delivery

被引：24
作者：
Gautier, Christelle
Cougnon, Charles
Pilard, Jean-Francois
Casse, Nathalie
Chenais, Benoit
Laulier, Marc
机构：
[1] Univ Maine, LCOM Chim Polymeres, UCO2M, CNRS UMR 6011, F-72085 Le Mans 9, France
[2] Univ Maine, Lab Biol & Genet Evolut, F-72085 Le Mans 9, France
来源：
BIOSENSORS & BIOELECTRONICS | 2007年 / 22卷 / 9-10期
关键词：
DNA sensor; label-free detection; DNA-modified polythiophene; electrochemical impedance spectroscopy; controlled delivery;
D O I：
10.1016/j.bios.2006.08.040
中图分类号：
Q6 [生物物理学];
学科分类号：
071011 ;
摘要：
A conducting polymer sensor for direct label-free DNA detection based on a polythiophene bearing an electroactive linker group is investigated. DNA hybridization is studied by electrochemical impedance spectroscopy (EIS) and quartz crystal microbalance (QCM) techniques. Modelling of DNA hybridization by EIS measurements exhibits the contribution of nucleic acid to a superficial p-doping process. A 675-mer single-stranded DNA is produced using asymmetric PCR from a DNA sequence of a transposable element mariner and hybridized to the previously immobilized probe. Electrochemical stimulus leads to the release "on demand" of DNA fragments and the amount delivery permits to do PCR amplification. (c) 2006 Elsevier B.V. All rights reserved.
引用
收藏
页码：2025 / 2031
页数：7
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