Bifunctional reporter genes: construction and expression in prokaryotic and eukaryotic cells

被引：7

作者：

Goldenkova, IV ^{[1
]}

Musiychuk, KA ^{[1
]}

Piruzian, ES ^{[1
]}

机构：

[1] Russian Acad Sci, Vavilov Inst Gen Genet, Moscow 119991, Russia

来源：

MOLECULAR BIOLOGY | 2003年 / 37卷 / 02期

基金：

俄罗斯基础研究基金会;

关键词：

reporter gene; licB; lichenase; green fluorescent protein; beta-glucuronidase; bifunctional proteins; prokaryotic and eukaryotic transformed cells;

D O I：

10.1023/A:1023310124842

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Bifunctional reporter proteins were constructed to combine Clostridium thermocellum lichenase (LicBM2) with Aequorea victoria green fluorescent protein (GFP) or with Escherichia coli beta-glucuronidase (GUS). The major properties of the initial proteins were preserved in the hybrid ones: LicBM2 was active at 65degreesC, GFP fluoresced, and GUS hydrolyzed its substrates. LicBM2 remained active after extension of its C or N end. Bifunctional reporter systems were shown to provide a convenient tool for studying the gene expression regulation in prokaryotic (E. coli) and eukaryotic (Saccharomyces cerevisiae, mammalian) cells, advantages of one reporter compensating for drawbacks of the other.

引用

页码：307 / 313

页数：7

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