Leptospira genomics

被引：9

作者：

Boursaux-Eude, C

St Girons, I

Zuerner, R

机构：

[1] Inst Pasteur, Unite Bacteriol Mol & Med, F-75724 Paris 15, France

[2] USDA ARS, Natl Anim Dis Ctr, Zoonot Dis Res Unit, Ames, IA 50010 USA

来源：

ELECTROPHORESIS | 1998年 / 19卷 / 04期

关键词：

chromosome organization; Leptospira; pulsed-field gel electrophoresis;

D O I：

10.1002/elps.1150190421

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The bacterial species Leptospira interrogans (sensu stricto) has a complex genome containing two circular chromosomal replicons. Comparative analysis of the larger chromosome reveals a fluid genetic organization with many large rearrangements differentiating two closely related strains. In the present study new genes were identified by partial sequence analysis of randomly cloned fragments of L. interrogans DNA. These genes were localized in regions of the genome by nucleic acid hybridization with DNA fragments separated by pulsed-field gel electrophoresis. The resulting genetic maps provide improved resolution for each strain and provide evidence for additional chromosomal rearrangements. Insertion elements may be involved in recombination events, as several are near regions of the chromosome that have undergone rearrangement.

引用

页码：589 / 592

页数：4

共 16 条

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