Optimization of in-situ monolithic synthesis for immunopreconcentration in capillary

被引：28

作者：

Faure, Karine ^{[1
]}

Delaunay, Nathalie ^{[1
]}

Alloncle, Guillaume ^{[1
]}

Cotte, Stephane ^{[1
]}

Rocca, Jean-Louis ^{[1
]}

机构：

[1] Univ Lyon 1, CNRS, UMR 5180, Sci Analyt Lab, F-69622 Villeurbanne, France

来源：

JOURNAL OF CHROMATOGRAPHY A | 2007年 / 1149卷 / 02期

关键词：

immunopreconcentration; monolith; capillary; ochratoxin A;

D O I：

10.1016/j.chroma.2007.03.031

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Poly(glycidyl methacrylate-co-ethylene dimethacrylate) monoliths have been synthetized in fused-silica capillary. The monomer mixture composition, initiation mode and porogen composition were optimized in order to provide a monolith with an homogeneous morphology and able to generate an electroosmotic flow via the incorporation of a small percentage of monomers possessing sulfonate group. Anti-ochratoxin A antibodies were immobilized through a single step on the epoxy groups leading to a miniaturized immunoextraction column. In order to evaluate the specificity of the analyte-antigen interaction on this immunosorbent, the retention of ochratoxin A was examined on this support but also on two complementary sorbents: one constituted by the non-bonded monolith and another one bonded with non-specific antibodies. Only the monolith bonded with anti-ochratoxin A antibodies lead to retention, showing the specificity of the interactions involved. This affinity phase based on a monolithic polymer support exhibits a high potential for specific preconcentration of small molecules. (c) 2007 Elsevier B.V. All rights reserved.

引用

页码：145 / 150

页数：6