Reporter proteins for in vivo fluorescence without oxygen

被引：300

作者：

Drepper, Thomas

Eggert, Thorsten

Circolone, Franco

Heck, Achim

Krauss, Ulrich

Guterl, Jan-Karl

Wendorff, Marion

Losi, Aba

Gaertner, Wolfgang

Jaeger, Karl-Erich

机构：

[1] Univ Dusseldorf, Res Ctr Julich, Inst Mol Enzyme Technol, D-52426 Julich, Germany

[2] EVOcatal GmbH, D-40225 Dusseldorf, Germany

[3] Univ Parma, Dept Phys, I-43100 Parma, Italy

[4] Max Planck Inst Bioinorgan Chem, D-45470 Mulheim, Germany

来源：

NATURE BIOTECHNOLOGY | 2007年 / 25卷 / 04期

关键词：

D O I：

10.1038/nbt1293

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Fluorescent reporter proteins such as green fluorescent protein are valuable noninvasive molecular tools for in vivo real- time imaging of living specimens. However, their use is generally restricted to aerobic systems, as the formation of their chromophores strictly requires oxygen. Starting with blue- light photoreceptors from Bacillus subtilis and Pseudomonas putida that contain light- oxygen- voltage - sensing domains, we engineered flavin mononucleotide - based fluorescent proteins that can be used as fluorescent reporters in both aerobic and anaerobic biological systems.

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收藏

页码：443 / 445

页数：3

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