Poor ovarian response to gonadotropin stimulation is associated with low expression of follicle-stimulating hormone receptor in granulosa cells

被引:85
作者
Cai, Jie [1 ]
Lou, Hang-ying [1 ]
Dong, Min-yue [1 ]
Lu, Xiu-e [1 ]
Zhu, Yi-min [1 ]
Gao, Hui-juan [1 ]
Huang, He-feng [1 ]
机构
[1] Zhejiang Univ, Womens Hosp, Sch Med, Dept Reprod Endocrinol, Hangzhou 310006, Peoples R China
基金
高等学校博士学科点专项科研基金;
关键词
FSH receptor; ovarian response; granulosa cell;
D O I
10.1016/j.fertnstert.2006.11.034
中图分类号
R71 [妇产科学];
学科分类号
100211 ;
摘要
Objective: To explore the importance of follicle-stimulating hormone receptor (FSHR) in granulosa cells in the ovarian response to gonadotropin stimulation. Design: Prospective study. Setting: A women's hospital in China. Patient(s): One hundred infertile women undergoing ovarian stimulation with recombinant follicle-stimulating hormone (rFSH). Intervention(s): These women were divided into three groups: poor, moderate, and high responders, according to the number of follicles with diameter >= 14 mm. The FSHR expression at both mRNA and protein levels was determined by either reverse transcription-polymerase chain reaction or Western blot in granulosa cells. E-2 concentrations in serum and FSH levels in serum/follicular fluid (FF) were measured by electrochemiluminescence immunoassay. Main Outcome Measure(S): Relative expression of mRNA and protein of FSHR in granulosa cells, serum E-2, FSH level in serum and FF, and the number of mature follicles. Result(S): The expression of FSHR, at both the mRNA and protein levels, was significantly different among the three groups, with the lowest expression in the poor responders. The level of FSHR protein was positively correlated with the peak level of serum E-2 and the number of mature oocytes. FSH levels in FF and the dosage of rFSH used were significantly different among the three groups, with the highest values in the poor responders. Conclusion(S): Different levels of FSHR expression in granulosa cells result in different ovarian response, and lower expression of FSHR may account for poor ovarian response to gonadotropin stimulation, which suggests the critical role of FSHR in the ovarian response to gonadotropin stimulation.
引用
收藏
页码:1350 / 1356
页数:7
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