The cytochrome bc1 and cytochrome c oxidase complexes associate to form a single supracomplex in yeast mitochondria

被引：210

作者：

Cruciat, CM

Brunner, S

Baumann, F

Neupert, W

Stuart, RA

机构：

[1] Marquette Univ, Dept Biol, Milwaukee, WI 53051 USA

[2] Univ Munich, Inst Physiol Chem, D-80336 Munich, Germany

来源：

JOURNAL OF BIOLOGICAL CHEMISTRY | 2000年 / 275卷 / 24期

关键词：

D O I：

10.1074/jbc.M001901200

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The mitochondrial electron transport chain complexes are large multisubunit complexes embedded in the inner membrane. We report here that in the yeast Saccharomyces cerevisiae, the cytochrome be, and cytochrome c oxidase complexes co-exist as a larger complex of similar to 1000 kDa in the mitochondrial membrane. Following solubilization with a mild detergent, the cytochrome bc(1)-cytochrome c oxidase complex remains stable. It was analyzed using the techniques of gel filtration and blue native-polyacrylamide gel electrophoresis. Direct physical. association of subunits of the cytochrome be, complex with those of the cytochrome c oxidase complex was verified by co-immunoprecipitation analysis. Our data indicate that the cytochrome be, complex is exclusively in association with the cytochrome c oxidase complex in yeast mitochondria. We term this complex the cytochrome bc(1)-cytochrome c oxidase supracomplex.

引用

页码：18093 / 18098

页数：6

共 31 条

[11] C- to N-terminal translocation of preproteins into mitochondria
Fölsch, H
Gaume, B
Brunner, M
Neupert, W
Stuart, RA
[J]. EMBO JOURNAL, 1998, 17 (22) : 6508 - 6515
[12] Hatefi, 1967, METHOD ENZYMOL, V10, P225
[13] Herrmann J.M., 1994, CELL BIOL, P538
[14] Resolution of the aerobic respiratory system of the thermoacidophilic archaeon, Sulfolobus sp strain 7 .1. The archaeal, terminal oxidase supercomplex is a functional fusion of respiratory complexes III and IV with no c-type cytochromes
Iwasaki, T
Matsuura, K
Oshima, T
[J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (52) : 30881 - 30892
[15] CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4
LAEMMLI, UK
[J]. NATURE, 1970, 227 (5259) : 680 - +
[16] The Saccharomyces cerevisiae NDE1 and NDE2 genes encode separate mitochondrial NADH dehydrogenases catalyzing the oxidation of cytosolic NADH
Luttik, MAH
Overkamp, KM
Kötter, P
de Vries, S
van Dijken, JP
Pronk, JT
[J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (38) : 24529 - 24534
[17] SUBUNIT-IV OF YEAST CYTOCHROME-C OXIDASE - CLONING AND NUCLEOTIDE SEQUENCING OF THE GENE AND PARTIAL AMINO-ACID SEQUENCING OF THE MATURE PROTEIN
MAARSE, AC
ADOLPHUS
VANLOON, PGM
RIEZMAN, H
GREGOR, I
SCHATZ, G
GRIVELL, LA
[J]. EMBO JOURNAL, 1984, 3 (12) : 2831 - 2837
[18] SHY1, the yeast homolog of the mammalian SURF-1 gene encodes a mitochondrial protein required for respiration
Mashkevich, G
Repetto, B
Glerum, DM
Jin, C
Tzagoloff, A
[J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (22) : 14356 - 14364
[19] PHILLIPS JD, 1990, J BIOL CHEM, V265, P20813
[20] PHILLIPS JD, 1993, J BIOL CHEM, V268, P11727

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