Homogeneous fluorescence-based DNA detection with water-soluble conjugated polymers

被引：578

作者：

Liu, B ^{[1
]}

Bazan, GC

机构：

[1] Univ Calif Santa Barbara, Dept Chem, Santa Barbara, CA 93106 USA

[2] Univ Calif Santa Barbara, Inst Polymers & Organ Solids, Dept Mat, Santa Barbara, CA 93106 USA

来源：

CHEMISTRY OF MATERIALS | 2004年 / 16卷 / 23期

关键词：

D O I：

10.1021/cm049587x

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Methods for real-time, high-sensitivity polynucleotide detection are of vast scientific and economic importance. Homogeneous biosensor assays that take advantage of the collective response of water-soluble conjugated polymers and the self-assembly characteristic of aqueous polyelectrolytes have shown potential for improvements over small molecule methods. Cationic polythiophenes transduce oligonucleotide hybridization into a colorimetric output based on conformational changes of the polymer upon interaction with single-stranded DNAs or double-stranded DNAs. Cationic poly(fluorene-co-phenylene) materials serve as donors in fluorescence energy-transfer assays which display signal amplification. Signal transduction in aqueous media is controlled by specific electrostatic interactions.

引用

页码：4467 / 4476

页数：10

共 77 条

[1]

[Anonymous], 1992, INTERMOLECULAR SURFA

[2] Conjugates of oligonucleotides with polyaromatic fluorophores as promising DNA probes [J].

Balakin, KV ;

Korshun, VA ;

Mikhalev, II ;

Maleev, GV ;

Malakhov, AD ;

Prokhorenko, IA ;

Berlin, YA .

BIOSENSORS & BIOELECTRONICS, 1998, 13 (7-8) :771-778

[3]

Bauerle P, 1998, ADV MATER, V10, P324, DOI 10.1002/(SICI)1521-4095(199803)10:4<324::AID-ADMA324>3.0.CO

[4]

2-E

[5] Interchain vs. intrachain energy transfer in acceptor-capped conjugated polymers [J].

Beljonne, D ;

Pourtois, G ;

Silva, C ;

Hennebicq, E ;

Herz, LM ;

Friend, RH ;

Scholes, GD ;

Setayesh, S ;

Müllen, K ;

Brédas, JL .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (17) :10982-10987

[6] Recognition of DNA topology in reactions between plasmid DNA and cationic copolymers [J].

Bronich, TK ;

Nguyen, HK ;

Eisenberg, A ;

Kabanov, AV .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2000, 122 (35) :8339-8343

[7] Semiconductor nanocrystals as fluorescent biological labels [J].

Bruchez, M ;

Moronne, M ;

Gin, P ;

Weiss, S ;

Alivisatos, AP .

SCIENCE, 1998, 281 (5385) :2013-2016

[8] DETECTION OF NUCLEIC-ACID HYBRIDIZATION BY NONRADIATIVE FLUORESCENCE RESONANCE ENERGY-TRANSFER [J].

CARDULLO, RA ;

AGRAWAL, S ;

FLORES, C ;

ZAMECNIK, PC ;

WOLF, DE .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (23) :8790-8794

[9] Enzyme-amplified amperometric detection of hybridization and of a single base pair mutation in an 18-base oligonucleotide on a 7-μm-diameter microelectrode [J].

Caruana, DJ ;

Heller, A .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1999, 121 (04) :769-774

[10] Single-molecule detection of specific nucleic acid sequences in unamplified genomic DNA [J].

Castro, A ;

Williams, JGK .

ANALYTICAL CHEMISTRY, 1997, 69 (19) :3915-3920

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