Homogeneous fluorescence-based DNA detection with water-soluble conjugated polymers

被引:578
作者
Liu, B [1 ]
Bazan, GC
机构
[1] Univ Calif Santa Barbara, Dept Chem, Santa Barbara, CA 93106 USA
[2] Univ Calif Santa Barbara, Inst Polymers & Organ Solids, Dept Mat, Santa Barbara, CA 93106 USA
关键词
D O I
10.1021/cm049587x
中图分类号
O64 [物理化学(理论化学)、化学物理学];
学科分类号
070304 ; 081704 ;
摘要
Methods for real-time, high-sensitivity polynucleotide detection are of vast scientific and economic importance. Homogeneous biosensor assays that take advantage of the collective response of water-soluble conjugated polymers and the self-assembly characteristic of aqueous polyelectrolytes have shown potential for improvements over small molecule methods. Cationic polythiophenes transduce oligonucleotide hybridization into a colorimetric output based on conformational changes of the polymer upon interaction with single-stranded DNAs or double-stranded DNAs. Cationic poly(fluorene-co-phenylene) materials serve as donors in fluorescence energy-transfer assays which display signal amplification. Signal transduction in aqueous media is controlled by specific electrostatic interactions.
引用
收藏
页码:4467 / 4476
页数:10
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