A novel lipopolysaccharide inducible C-C chemokine receptor related gene in murine macrophages

被引：47

作者：

Shimada, T

Matsumoto, M

Tatsumi, Y

Kanamaru, A

Akira, S

机构：

[1] Hyogo Coll Med, Dept Biochem, Nishinomiya, Hyogo 663, Japan

[2] Kinki Univ, Sch Med, Dept Internal Med 3, Osaka 589, Japan

来源：

FEBS LETTERS | 1998年 / 425卷 / 03期

关键词：

lipopolysaccharide; macrophage; chemokine receptor; gene expression regulation; subtraction technique; inflammation;

D O I：

10.1016/S0014-5793(98)00299-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

To identify genes induced in activated macrophages, we screened a cDNA Library prepared from the lipopolysaccharide (LPS)-treated cell line, RAW261, using the suppression subtractive hybridization technique. One of the clones isolated was dramatically induced by LPS in macrophages. The predicted protein sequence of this gene contains the domain unique to seven transmembrane receptors, and shows similarity with mouse C-C chemokine receptor 5 (CCR5), Therefore, we designated it LPS inducible C-C chemokine receptor related gene (L-CCR), Northern blot analysis revealed that L-CCR was specifically expressed in differentiated macrophages after LPS stimulation. These results show that L-CCR is a novel C-C chemokine receptor related gene induced by LPS in macrophages and may play an important role in inflammatory responses. (C) 1998 Federation of European Biochemical Societies.

引用

页码：490 / 494

页数：5