Parallel multispot detection of target hybridization to surface-bound probe oligonucleotides of different base mismatch by surface-plasmon field-enhanced fluorescence microscopy

被引：31

作者：

Liebermann, T ^{[1
]}

Knoll, W ^{[1
]}

机构：

[1] Max Planck Inst Polymer Res, D-55128 Mainz, Germany

来源：

LANGMUIR | 2003年 / 19卷 / 05期

关键词：

D O I：

10.1021/la026263j

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We introduce surface-plasmon field-enhanced fluorescence microscopy for the on-line determination of hybridization reactions between targets from solution to complementary probe oligonucleotides which are surface-bound to a sensor substrate in a matrix format. This way, the parallel and real-time detection of the association and dissociation kinetics (rate constants) of hybrids of different mismatch situations can be quantitatively analyzed.

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页码：1567 / 1572

页数：6

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