Visual detection of pandemic influenza A H1N1 Virus 2009 by reverse-transcription loop-mediated isothermal amplification with hydroxynaphthol blue dye

被引：80

作者：

Ma, Xue-jun ^{[1
]}

Shu, Yue-long ^{[1
]}

Nie, Kai ^{[1
]}

Qin, Meng ^{[1
]}

Wang, Da-yan ^{[1
]}

Gao, Rong-bao ^{[1
]}

Wang, Miao ^{[1
]}

Wen, Le-ying ^{[1
]}

Han, Feng ^{[1
]}

Zhou, Shu-mei ^{[1
]}

Zhao, Xiang ^{[1
]}

Cheng, Yan-hui ^{[1
]}

Li, De-xin ^{[1
]}

Dong, Xiao-ping ^{[1
]}

机构：

[1] Chinese Ctr Dis Control & Prevent, Natl Inst Viral Dis Control & Prevent, State Key Lab Infect Dis Control & Prevent, Beijing 100052, Peoples R China

来源：

JOURNAL OF VIROLOGICAL METHODS | 2010年 / 167卷 / 02期

关键词：

Pandemic influenza A H1N1 virus; Reverse-transcription loop-mediated isothermal amplification; Colorimetric detection; REAL-TIME DETECTION; RAPID DETECTION; ASSAY;

D O I：

10.1016/j.jviromet.2010.03.027

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A sensitive reverse-transcription loop-mediated Isothermal amplification (RT-LAMP) assay was developed for rapid visual detection of pandemic influenza A Hi NI virus infection The reaction was performed in one step in a single tube at 65 degrees C for 60 mm with the addition of hydroxynaphthol blue (HNB) dye prior to amplification The detection limit of the RI-LAMP assay was approximately 60 copies, and no cross-detection was observed The assay was evaluated further with 50 clinical specimens diagnosed clinically with seasonal influenza or pandemic influenza A H1N1 virus infection RT-LAMP with HNB dye was demonstrated to be a sensitive and easy assay for rapid detection of pandemic influenza A Hi NI virus (C)2010 Elsevier B V All rights reserved

引用

页码：214 / 217

页数：4

共 12 条

[1]

Anonymous, 2008, Chinese Journal of Virology, V24, P178

[2] Rapid detection of HIV-1 by reverse-transcription, loop-mediated isothermal amplification (RT-LAMP) [J].