Prevotella intermedia lipopolysaccharide stimulates release of nitric oxide by inducing expression of inducible nitric oxide synthase

被引:44
作者
Kim, SJ
Ha, MS
Choi, EY
Choi, JI
Choi, IS
机构
[1] Pusan Natl Univ, Coll Dent, Dept Periodontol, Pusan 602739, South Korea
[2] Silla Univ, Coll Nat Sci, Dept Life Sci, Pusan, South Korea
关键词
inducible nitric oxide synthase; nitric oxide; lipopolysaccharide; Prevotella intermedia;
D O I
10.1111/j.1600-0765.2004.00757.x
中图分类号
R78 [口腔科学];
学科分类号
1003 ;
摘要
Objectives: The purpose of this study was to examine the effects of lipopolysaccharide from Prevotella intermedia, a major cause of inflammatory periodontal disease, on the production of nitric oxide (NO) and expression of inducible nitric oxide synthase (iNOS) in the murine macrophage cell line RAW264.7. We also attempted to throw light on the signaling mechanisms involved in P. intermedia lipopolysaccharide-induced NO production. Material and methods: Lipopolysaccharide from P. intermedia ATCC 25611 was prepared by the standard hot phenol-water method. NO production was assayed by measuring the accumulation of nitrite in culture supernatants. Western blot analysis of iNOS and analysis of reverse transcription-polymerase chain reaction (RT-PCR) products were carried out. Results: We found that P. intermedia lipopolysaccharide can induce iNOS expression and stimulate the release of NO without additional stimuli and demonstrated an important role of the transcription factor nuclear factor-kappaB (NF-kappaB) and microtubule polymerization in NO production. The production of NO required L-arginine but not activation of protein kinase C or protein tyrosine kinase. Conclusions: The present study clearly shows that P. intermedia lipopolysaccharide fully induced iNOS expression and NO production in RAW264.7 cells in the absence of other stimuli. The ability of P. intermedia lipopolysaccharide to promote the production of NO may be important in the pathogenesis of inflammatory periodontal disease.
引用
收藏
页码:424 / 431
页数:8
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