Comparison of poultry processing equipment surfaces for susceptibility to bacterial attachment and biofilm formation

被引:56
作者
Arnold, JW [1 ]
Silvers, S [1 ]
机构
[1] USDA ARS, Russell Res Ctr, Athens, GA 30604 USA
关键词
biofilm; bacteria; pathogen; poultry processing; stainless steel;
D O I
10.1093/ps/79.8.1215
中图分类号
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号
0905 ;
摘要
During processing of poultry meat products, broiler carcasses come in contact with many solid surfaces. Bacteria from the carcasses can attach to wet equipment surfaces, form biofilms, and provide a source of cross-contamination for subsequent carcasses. In this study an array of common equipment surface materials was compared for susceptibility to bacterial attachment and biofilms. To model mixed microbial populations relevant to poultry processing, samples were taken directly from the processing line and exposed to the surface materials. Whole carcasses were rinsed with phosphate-buffered saline (100 mL), and the rinse was diluted in nutrient broth. Absorbance values (412 nm) of the suspensions at varying dilutions containing test surfaces were compared hourly with controls without test surfaces. The kinetics of bacterial attachment and biofilm formation on test surfaces were determined under the influence of pH, time, and bacterial cell density, and the elemental composition of the surface materials was determined by energy-dispersive X-ray analysis. Our results showed that surfaces vary in affinity for bacterial attachment and biofilm formation. Analysis by spectrophotometry and scanning electron microscopy confirmed that attachment to stainless steel, polyethylene, and belting was not significantly different from controls. Attachment to picker-finger rubber was significantly less than attachment to stainless steel and the other surfaces. In fact, picker-finger rubber inhibits bacterial contamination. An increased understanding of bacterial attachment and biofilm formation will assist in the development of interventions to counteract these processes and, thereby, enhance plant sanitation and pathogen control.
引用
收藏
页码:1215 / 1221
页数:7
相关论文
共 42 条
[1]   INCIDENCE AND PROPERTIES OF STAPHYLOCOCCUS-AUREUS ASSOCIATED WITH TURKEYS DURING PROCESSING AND FURTHER-PROCESSING OPERATIONS [J].
ADAMS, BW ;
MEAD, GC .
JOURNAL OF HYGIENE, 1983, 91 (03) :479-490
[2]   CELL-SURFACE PROPERTIES CORRELATED WITH COHESION IN MYXOCOCCUS-XANTHUS [J].
ARNOLD, JW ;
SHIMKETS, LJ .
JOURNAL OF BACTERIOLOGY, 1988, 170 (12) :5771-5777
[3]   INHIBITION OF CELL-CELL INTERACTIONS IN MYXOCOCCUS-XANTHUS BY CONGO RED [J].
ARNOLD, JW ;
SHIMKETS, LJ .
JOURNAL OF BACTERIOLOGY, 1988, 170 (12) :5765-5770
[4]   Growth of Listeria monocytogenes as a biofilm on various food-processing surfaces [J].
Blackman, IC ;
Frank, JF .
JOURNAL OF FOOD PROTECTION, 1996, 59 (08) :827-831
[5]   BACTERIAL BIOFILMS IN NATURE AND DISEASE [J].
COSTERTON, JW ;
CHENG, KJ ;
GEESEY, GG ;
LADD, TI ;
NICKEL, JC ;
DASGUPTA, M ;
MARRIE, TJ .
ANNUAL REVIEW OF MICROBIOLOGY, 1987, 41 :435-464
[6]   SUSCEPTIBILITY OF SUSPENDED AND SURFACE-ATTACHED SALMONELLA-ENTERITIDIS TO BIOCIDES AND ELEVATED-TEMPERATURES [J].
DHIR, VK ;
DODD, CER .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1995, 61 (05) :1731-1738
[7]   DETECTION OF THE SITE OF CONTAMINATION BY STAPHYLOCOCCUS-AUREUS WITHIN THE DEFEATHERING MACHINERY OF A POULTRY-PROCESSING PLANT [J].
DODD, CER ;
MEAD, GC ;
WAITES, WM .
LETTERS IN APPLIED MICROBIOLOGY, 1988, 7 (03) :63-66
[8]   Determination of the principal sources of Listeria spp contamination in poultry meat and a poultry processing plant [J].
Franco, CM ;
Quinto, EJ ;
Fente, C ;
RodriguezOtero, JL ;
Dominguez, L ;
Cepeda, A .
JOURNAL OF FOOD PROTECTION, 1995, 58 (12) :1320-1325
[9]   SURFACE-ADHERENT GROWTH OF LISTERIA-MONOCYTOGENES IS ASSOCIATED WITH INCREASED RESISTANCE TO SURFACTANT SANITIZERS AND HEAT [J].
FRANK, JF ;
KOFFI, RA .
JOURNAL OF FOOD PROTECTION, 1990, 53 (07) :550-554
[10]   DISTRIBUTION OF STAPHYLOCOCCUS-AUREUS IN A POULTRY-PROCESSING PLANT [J].
GIBBS, PA ;
PATTERSON, JT ;
THOMPSON, JK .
JOURNAL OF APPLIED BACTERIOLOGY, 1978, 44 (03) :401-410