Modeling Development and Disease with Organoids

被引：2394

作者：

Clevers, Hans ^{[1
,2
]}

机构：

[1] Royal Netherlands Acad Arts & Sci, Princess Maxima Ctr, Hubrecht Inst, NL-3584 CT Utrecht, Netherlands

[2] Univ Med Ctr Utrecht, NL-3584 CT Utrecht, Netherlands

来源：

CELL | 2016年 / 165卷 / 07期

关键词：

PLURIPOTENT STEM-CELLS; IN-VITRO EXPANSION; DUCTAL PANCREATIC-CANCER; EPITHELIAL-CELLS; CYSTIC-FIBROSIS; SMALL-INTESTINE; DIRECTED DIFFERENTIATION; ALVEOLAR CELLS; MAMMARY-GLAND; SINGLE LGR5;

D O I：

10.1016/j.cell.2016.05.082

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

Recent advances in 3D culture technology allow embryonic and adult mammalian stem cells to exhibit their remarkable self-organizing properties, and the resulting organoids reflect key structural and functional properties of organs such as kidney, lung, gut, brain and retina. Organoid technology can therefore be used to model human organ development and various human pathologies 'n a dish.'' Additionally, patient-derived organoids hold promise to predict drug response in a personalized fashion. Organoids open up new avenues for regenerative medicine and, in combination with editing technology, for gene therapy. The many potential applications of this technology are only beginning to be explored.

引用

页码：1586 / 1597

页数：12

共 91 条

[1]

Generation of functional thyroid from embryonic stem cells [J].

Antonica, Francesco ;

Kasprzyk, Dominika Figini ;

Opitz, Robert ;

Iacovino, Michelina ;

Liao, Xiao-Hui ;

Dumitrescu, Alexandra Mihaela ;

Refetoff, Samuel ;

Peremans, Kathelijne ;

Manto, Mario ;

Kyba, Michael ;

Costagliola, Sabine .

NATURE, 2012, 491 (7422) :66-U170

[2]

Type 2 alveolar cells are stem cells in adult lung [J].

Barkauskas, Christina E. ;

Cronce, Michael J. ;

Rackley, Craig R. ;

Bowie, Emily J. ;

Keene, Douglas R. ;

Stripp, Barry R. ;

Randell, Scott H. ;

Noble, Paul W. ;

Hogan, Brigid L. M. .

JOURNAL OF CLINICAL INVESTIGATION, 2013, 123 (07) :3025-3036

[3]

Identification of stem cells in small intestine and colon by marker gene Lgr5 [J].

Barker, Nick ;

van Es, Johan H. ;

Kuipers, Jeroen ;

Kujala, Pekka ;

van den Born, Maaike ;

Cozijnsen, Miranda ;

Haegebarth, Andrea ;

Korving, Jeroen ;

Begthel, Harry ;

Peters, Peter J. ;

Clevers, Hans .

NATURE, 2007, 449 (7165) :1003-U1

[4]

Lgr5+ve Stem Cells Drive Self-Renewal in the Stomach and Build Long-Lived Gastric Units In Vitro [J].

Barker, Nick ;

Huch, Meritxell ;

Kujala, Pekka ;

van de Wetering, Marc ;

Snippert, Hugo J. ;

van Es, Johan H. ;

Sato, Toshiro ;

Stange, Daniel E. ;

Begthel, Harry ;

van den Born, Maaike ;

Danenberg, Esther ;

van den Brink, Stieneke ;

Korving, Jeroen ;

Abo, Arie ;

Peters, Peter J. ;

Wright, Nick ;

Poulsom, Richard ;

Clevers, Hans .

CELL STEM CELL, 2010, 6 (01) :25-36

[5]

In Vitro Expansion of Human Gastric Epithelial Stem Cells and Their Responses to Bacterial Infection [J].

Bartfeld, Sina ;

Bayram, Tulay ;

van de Wetering, Marc ;

Huch, Meritxell ;

Begthel, Harry ;

Kujala, Pekka ;

Vries, Robert ;

Peters, Peter J. ;

Clevers, Hans .

GASTROENTEROLOGY, 2015, 148 (01) :126-U554

[6]

Organoid Models of Human and Mouse Ductal Pancreatic Cancer [J].

Boj, Sylvia F. ;

Hwang, Chang-Il ;

Baker, Lindsey A. ;

Chio, Iok In Christine ;

Engle, Dannielle D. ;

Corbo, Vincenzo ;

Jager, Myrthe ;

Ponz-Sarvise, Mariano ;

Tiriac, Herve ;

Spector, Mona S. ;

Gracanin, Ana ;

Oni, Tobiloba ;

Yu, Kenneth H. ;

van Boxtel, Ruben ;

Huch, Meritxell ;

Rivera, Keith D. ;

Wilson, John P. ;

Feigin, Michael E. ;

Oehlund, Daniel ;

Handly-Santana, Abram ;

Ardito-Abraham, Christine M. ;

Ludwig, Michael ;

Elyada, Ela ;

Alagesan, Brinda ;

Biffi, Giulia ;

Yordanov, Georgi N. ;

Delcuze, Bethany ;

Creighton, Brianna ;

Wright, Kevin ;

Park, Youngkyu ;

Morsink, Folkert H. M. ;

Molenaar, I. Quintus ;

Rinkes, Inne H. Borel ;

Cuppen, Edwin ;

Hao, Yuan ;

Jin, Ying ;

Nijman, Isaac J. ;

Iacobuzio-Donahue, Christine ;

Leach, Steven D. ;

Pappin, Darryl J. ;

Hammell, Molly ;

Klimstra, David S. ;

Basturk, Olca ;

Hruban, Ralph H. ;

Offerhaus, George Johan ;

Vries, Robert G. J. ;

Clevers, Hans ;

Tuveson, David A. .

CELL, 2015, 160 (1-2) :324-338

[7]

Human cerebral organoids recapitulate gene expression programs of fetal neocortex development [J].

Camp, J. Gray ;

Badsha, Farhath ;

Florio, Marta ;

Kanton, Sabina ;

Gerber, Tobias ;

Wilsch-Braeuninger, Michaela ;

Lewitus, Eric ;

Sykes, Alex ;

Hevers, Wulf ;

Lancaster, Madeline ;

Knoblich, Juergen A. ;

Lachmann, Robert ;

Paeaebo, Svante ;

Huttner, Wieland B. ;

Treutlein, Barbara .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2015, 112 (51) :15672-15677

[8]

Human Pluripotent Stem Cell Culture: Considerations for Maintenance, Expansion, and Therapeutics [J].

Chen, Kevin G. ;

Mallon, Barbara S. ;

McKay, Ronald D. G. ;

Robey, Pamela G. .

CELL STEM CELL, 2014, 14 (01) :13-26

[9]

Reprogramming Cellular Identity for Regenerative Medicine [J].

Cherry, Anne B. C. ;

Daley, George Q. .

CELL, 2012, 148 (06) :1110-1122

[10]

Single luminal epithelial progenitors can generate prostate organoids in culture [J].

Chua, Chee Wai ;

Shibata, Maho ;

Lei, Ming ;

Toivanen, Roxanne ;

Barlow, LaMont J. ;

Bergren, Sarah K. ;

Badani, Ketan K. ;

Mc Kiernan, James M. ;

Benson, Mitchell C. ;

Hibshoosh, Hanina ;

Shen, Michael M. .

NATURE CELL BIOLOGY, 2014, 16 (10) :951-961

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