Immunoreactivity and in-vitro effect on human sperm-egg binding of antibodies against peptides corresponding to bonnet monkey zona pellucida-3 glycoprotein

被引：37

作者：

Afzalpurkar, A

Shibahara, H

Hasegawa, A

Koyama, K

Gupta, SK ^{[1
]}

机构：

[1] Natl Inst Immunol, Gamete Antigen Lab, New Delhi 110067, India

[2] Hyogo Coll Med, Dept Obstet & Gynaecol, Nishinomiya, Hyogo, Japan

来源：

HUMAN REPRODUCTION | 1997年 / 12卷 / 12期

关键词：

anti-peptide antibodies; in-vitro contraception; native protein reactivity; synthetic peptides; zona pellucida;

D O I：

10.1093/humrep/12.12.2664

中图分类号：

R71 [妇产科学];

学科分类号：

100211 ;

摘要：

The following synthetic peptides were made as immunogens for development of a zona-based contraceptive vaccine: P1, KQPFWLLQGGASRAETSVQPVLVE [amino acids (aa) 23-45 with an additional K at the N-terminus]; P2, FSEEKLVFSLRLMEENC (aa 164-179 with an additional C at the C-terminus and T-170 replaced by V); and P3, CSFSKSSNSWFPVEGPADICQCC (aa 300-322). The aa are numbered on the basis of bonnet monkey ZP3 precursor protein, Antibodies against an additional peptide P4, KGDCGTPSHSRRQPHVVSQWSRSA (aa 324-347), significantly inhibits human sperm-oocyte binding, In addition, antibodies against cocktail of peptide-diphtheria toroid conjugates also significantly inhibit the binding of spermatozoa to zona pellucida in a hemizona assay, These results will further help in the design of an immunocontraceptive vaccine based upon synthetic peptides corresponding to ZP3.

引用

页码：2664 / 2670

页数：7

共 31 条

[1] Induction of native protein reactive antibodies by immunization with peptides containing linear B-cell epitopes defined by anti-porcine ZP3 beta monoclonal antibodies [J].