A novel yeast protein showing specific association with the cyclin-dependent kinase 5

被引：5

作者：

Huang, QQ

Lee, KY

Wang, JH

机构：

[1] UNIV CALGARY,DEPT MED BIOCHEM,CALGARY,AB T2N 4N1,CANADA

[2] UNIV CALGARY,MRC,GRP SIGNAL TRANSDUCT,CALGARY,AB T2N 4N1,CANADA

来源：

FEBS LETTERS | 1996年 / 378卷 / 01期

基金：

英国医学研究理事会;

关键词：

Cdk5; neuronal Cdk5 activator (nck5a); yeast;

D O I：

10.1016/0014-5793(95)01394-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The present study describes a significant amino acid sequence homology between neuronal Cdk5 activator (nck5a) and an open reading frame of an unknown gene on the yeast S. cerevisiae chromosome III. A cDNA encoding a 25 kDa fragment of this yeast protein, the region containing homologous sequence to nck5a was cloned and expresed in E. coli as a glutathine-S-transferase fusion protein (GST-p25Y), GST-p25Y was found to block the in vitro activation of Cdk5 by nck5a and to affinity precipitate Cdk5 from bovine brain extract. The observations indicate that the yeast protein is capable of specific and high affinity association with Cdk5.

引用

页码：48 / 50

页数：3

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