Sensitive bondforce measurements of ligand-receptor pairs with magnetic beads

被引：24

作者：

Panhorst, M ^{[1
]}

Kamp, PB ^{[1
]}

Reiss, G ^{[1
]}

Brückl, H ^{[1
]}

机构：

[1] Univ Bielefeld, D-33615 Bielefeld, Germany

来源：

BIOSENSORS & BIOELECTRONICS | 2005年 / 20卷 / 08期

关键词：

magnetic beads; bondforce; cooperative binding; streptavidin; avidin; biotin;

D O I：

10.1016/j.bios.2004.06.030

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

The bondforces between biotinylated surfaces and streptavidin or avidin coated beads are investigated by a magnetic field based manipulation system for magnetic microbeads. The magnetic field is generated by currents through a set of conducting lines, and its gradient exerts a force onto the magnetic beads. The force can be increased until the bond between the bead and the surface breaks. Consistent with other groups we found two conformations for both investigated bonds. The measured bondforces for the two conformations are for Streptavidin-Biotin: 55.9 and 244.7 fN and for Avidin-Biotin: 15.9 and 58.4 fN. These very low bondforces (10-100 times smaller than earlier measurements) match to the extremely low loading rate of about 1 fN/s. This new technique thus allows to investigate biomolecular bonds by extremely low forces. (C) 2004 Elsevier B.V. All rights reserved.

引用

页码：1685 / 1689

页数：5

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