Analysis of gene expression profiles between apical papilla tissues, stem cells from apical papilla and cell sheet to identify the key modulators in MSCs niche

被引：22

作者：

Diao, Shu ^{[1
,2
]}

Lin, Xiao ^{[1
,3
]}

Wang, Liping ^{[1
]}

Dong, Rui ^{[1
]}

Du, Juan ^{[1
,4
]}

Yang, Dongmei ^{[1
,2
]}

Fan, Zhipeng ^{[1
]}

机构：

[1] Capital Med Univ, Sch Stomatol, Beijing Key Lab Tooth Regenerat & Funct Reconstru, Lab Mol Signaling & Stem Cells Therapy, Beijing, Peoples R China

[2] Capital Med Univ, Sch Stomatol, Dept Pediat Dent, Beijing, Peoples R China

[3] Capital Med Univ, Sch Stomatol, Dept Implant Dent, Beijing, Peoples R China

[4] Capital Med Univ, Sch Stomatol, Beijing Key Lab Tooth Regenerat & Funct Reconstru, Mol Lab Gene Therapy & Tooth Regenerat, Beijing, Peoples R China

来源：

CELL PROLIFERATION | 2017年 / 50卷 / 03期

基金：

中国国家自然科学基金;

关键词：

OSTEOGENIC DIFFERENTIATION; SELF-RENEWAL; IN-VITRO; PROLIFERATION; BIOLOGY; GROWTH; MULTIPOTENCY; TEETH; MICE;

D O I：

10.1111/cpr.12337

中图分类号：

Q2 [细胞生物学];

学科分类号：

071013 [干细胞生物学];

摘要：

Objectives: The microenvironmental niche plays the key role for maintaining the cell functions. The stem cells from apical papilla (SCAPs) are important for tooth development and regeneration. However, there is limited knowledge about the key factors in niche for maintaining the function of SCAPs. In this study, we analyse the gene expression profiles between apical papilla tissues, SCAPs and SCAPs cell sheet to identify the key genes in SCAPs niche. Materials and methods: Microarray assays and bioinformatic analysis were performed to screen the differential genes between apical papilla tissues and SCAPs, and SCAPs and SCAPs cell sheet. Recombinant human BMP6 protein was used in SCAPs. Then CCK-8 assay, CFSE assay, alkaline phosphatase activity, alizarin red staining, quantitative calcium analysis and real-time reverse transcriptase-polymerase chain reaction were performed to investigate the cell proliferation and differentiation potentials of SCAPs. Results: Microarray analysis found that 846 genes were up-regulated and 1203 genes were down-regulated in SCAPs compared with apical papilla tissues. While 240 genes were up-regulated and 50 genes were down-regulated in SCAPs compared to in SCAPs cell sheet. Moreover, only 31 gene expressions in apical papilla tissues were recovered in cell sheet compared with SCAPs. Bioinformatic analysis identified that TGF-beta, WNT and MAPK signalling pathways may play an important role in SCAPs niche. Based on the analysis, we identified one key growth factor in niche, BMP6, which could enhance the cell proliferation, the osteo/dentinogenic, neurogenic and angiogenic differentiation potentials of SCAPs. Conclusions: Our results provided insight into the mechanisms of the microenvironmental niche which regulate the function of SCAPs, and identified the key candidate genes in niche to promote mesenchymal stem cells-mediated dental tissue regeneration.

引用

页数：9

共 52 条

[1]

Anusaksathien Orasa, 2002, Current Pharmaceutical Biotechnology, V3, P129, DOI 10.2174/1389201023378391

[2]

Gene Ontology: tool for the unification of biology [J].

Ashburner, M ;

Ball, CA ;

Blake, JA ;

Botstein, D ;

Butler, H ;

Cherry, JM ;

Davis, AP ;

Dolinski, K ;

Dwight, SS ;

Eppig, JT ;

Harris, MA ;

Hill, DP ;

Issel-Tarver, L ;

Kasarskis, A ;

Lewis, S ;

Matese, JC ;

Richardson, JE ;

Ringwald, M ;

Rubin, GM ;

Sherlock, G .

NATURE GENETICS, 2000, 25 (01) :25-29

[3]

Vascular smooth muscle cell expression of ectonucleotidase CD39 (ENTPD1) is required for neointimal formation in mice [J].

Behdad, Amir ;

Sun, Xiaofeng ;

Khalpey, Zain ;

Enjyoji, Keiichi ;

Wink, Marcia ;

Wu, Yan ;

Usheva, Anny ;

Robson, Simon C. .

PURINERGIC SIGNALLING, 2009, 5 (03) :335-342

[4]

Optimization of in vitro expansion of human multipotent mesenchymal stromal cells for cell-therapy approaches: Further insights in the search for a fetal calf serum substitute [J].

Bernardo, M. E. ;

Avanzini, M. A. ;

Perotti, C. ;

Cometa, A. M. ;

Moretta, A. ;

Lenta, E. ;

Del Fante, C. ;

Novara, F. ;

De Silvestri, A. ;

Amendola, G. ;

Zuffardi, O. ;

Maccario, R. ;

Locatelli, F. .

JOURNAL OF CELLULAR PHYSIOLOGY, 2007, 211 (01) :121-130

[5]

Self-renewal, multipotency, and the existence of two cell populations within an epithelial stem cell niche [J].

Blanpain, C ;

Lowry, WE ;

Geoghegan, A ;

Polak, L ;

Fuchs, E .

CELL, 2004, 118 (05) :635-648

[6]

Effect of surgical implantation of recombinant human bone morphogenetic protein-2 in a bioabsorbable collagen sponge or calcium phosphate putty carrier in intrabony periodontal defects in the baboon [J].

Blumenthal, NM ;

Koh-Kunst, G ;

Alves, MEAF ;

Miranda, D ;

Sorensen, RG ;

Wozney, JM ;

Wikesjö, UME .

JOURNAL OF PERIODONTOLOGY, 2002, 73 (12) :1494-1506

[7]

Human mesenchymal stem cell proliferation and osteogenic differentiation in fibrin gels in vitro [J].

Catelas, Isabelle ;

Sese, Nadjah ;

Wu, Benjamin M. ;

Dunn, James C. Y. ;

Helgerson, Sam ;

Tawil, Bill .

TISSUE ENGINEERING, 2006, 12 (08) :2385-2396

[8]

PDGFB-based stem cell gene therapy increases bone strength in the mouse [J].

Chen, Wanqiu ;

Baylink, David J. ;

Brier-Jones, Justin ;

Neises, Amanda ;

Kiroyan, Jason B. ;

Rundle, Charles H. ;

Lau, Kin-Hing William ;

Zhang, Xiao-Bing .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2015, 112 (29) :E3893-E3900

[9]

Osteogenic activity of the fourteen types of human bone morphogenetic proteins (BMPs) [J].

Cheng, HW ;

Jiang, W ;

Phillips, FM ;

Haydon, RC ;

Peng, Y ;

Zhou, L ;

Luu, HH ;

An, NL ;

Breyer, B ;

Vanichakarn, P ;

Szatkowski, JP ;

Park, JY ;

He, TC .

JOURNAL OF BONE AND JOINT SURGERY-AMERICAN VOLUME, 2003, 85A (08) :1544-1552

[10]

THE C-FOS PROTEIN INTERACTS WITH C-JUN/AP-1 TO STIMULATE TRANSCRIPTION OF AP-1 RESPONSIVE GENES [J].

CHIU, R ;

BOYLE, WJ ;

MEEK, J ;

SMEAL, T ;

HUNTER, T ;

KARIN, M .

CELL, 1988, 54 (04) :541-552

← 1 2 3 4 5 6 →