Cross-linked Candida antarctica lipase B is active in denaturing ionic liquids

被引：104

作者：

Toral, Antonio Ruiz

de los Rios, Antonia P.

Hernandez, Francisco J.

Janssen, Michiel H. A.

Schoevaart, Rob

van Rantwijk, Fred

Sheldon, Roger A.

机构：

[1] Delft Univ Technol, Lab Biocatalysis & Organ Chem, NL-2628 BL Delft, Netherlands

[2] CLEA Technol, NL-2628 BL Delft, Netherlands

来源：

ENZYME AND MICROBIAL TECHNOLOGY | 2007年 / 40卷 / 05期

关键词：

Candida antarctica lipase B; cross-linking; ionic liquids; immobilisation; 1-butyl-3-methylimidazolium nitrate; alcohol resolution; amine resolution;

D O I：

10.1016/j.enzmictec.2006.08.027

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Candida antarctica lipase 13, adsorbed and cross-linked on a polypropylene carrier, maintained its activity when dispersed in ionic liquids, such as [BMIm][NO3], that denatured the free enzyme. The acylations (kinetic resolutions) of I -phenylethanol (E > 500) and I-phenylethylamine (E= 95), when carried out in [BMIm][NO3], were slow in comparison with molecular solvents but highly enantioselective. (c) 2006 Elsevier Inc. All rights reserved.

引用

收藏

页码：1095 / 1099

页数：5

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