Lesion simulating disease 1 -: Is required for acclimation to conditions that promote excess excitation energy

被引:255
作者
Mateo, A
Mühlenbock, P
Rustérucci, C
Chang, CCC
Miszalski, Z
Karpinska, B
Parker, JE
Mullineaux, PM
Karpinski, S [1 ]
机构
[1] Stockholm Univ, Dept Bot, SE-10691 Stockholm, Sweden
[2] Umea Univ, Dept Plant Physiol, SE-90185 Umea, Sweden
[3] Umea Plant Sci Ctr, SE-90185 Umea, Sweden
[4] Univ Essex, Dept Biol Sci, Colchester CO4 3SQ, Essex, England
[5] Max Planck Inst Plant Breeding Res, Dept Plant Microbe Interact, D-50829 Cologne, Germany
[6] Polish Acad Sci, Inst Plant Physiol, PL-30239 Krakow, Poland
关键词
D O I
10.1104/pp.104.043646
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
The lsd1 mutant of Arabidopsis fails to limit the boundaries of hypersensitive cell death response during avirulent pathogen infection and initiates unchecked lesions in long day photoperiod. giving rise to the runaway cell death (rcd) phenotype. We link here the initiation and propagation of rcd to the activity of photosystem II, stomatal conductance and ultimately to photorespiratory H2O2. A cross of lsd1 with the chlorophyll a/b binding harvesting-organelle specific (designated cao) mutant, which has a reduced photosystem II antenna, led to reduced lesion formation in the lsd1/cao double mutant. This lsd1 mutant also had reduced stomatal conductance and catalase activity in short-day permissive conditions and induced H2O2 accumulation followed by rcd when stomatal gas exchange was further impeded. All of these traits depended on the defense regulators EDS1 and PAD4. Furthermore, nonphotorespiratory conditions retarded propagation of lesions in lsd1. These data suggest that lsd1 failed to acclimate to light conditions that promote excess excitation energy (EEE) and that LSD1 function was required for optimal catalase activity. Through this regulation LSD1 can influence the effectiveness of photorespiration in dissipating EEE and consequently may be a key determinant of acclimatory processes. Salicylic acid, which induces stomatal closure, inhibits catalase activity and triggers the rcd phenotype in lsd1, also impaired acclimation of wild-type plants to conditions that promote EEE. We propose that the roles of LSD1 in light acclimation and in restricting pathogen-induced cell death are functionally linked.
引用
收藏
页码:2818 / 2830
页数:13
相关论文
共 82 条
[1]  
AEBI H, 1984, METHOD ENZYMOL, V105, P121
[3]   Reactive oxygen intermediates mediate a systemic signal network in the establishment of plant immunity [J].
Alvarez, ME ;
Pennell, RI ;
Meijer, PJ ;
Ishikawa, A ;
Dixon, RA ;
Lamb, C .
CELL, 1998, 92 (06) :773-784
[4]   The water-water cycle in chloroplasts: Scavenging of active oxygens and dissipation of excess photons [J].
Asada, K .
ANNUAL REVIEW OF PLANT PHYSIOLOGY AND PLANT MOLECULAR BIOLOGY, 1999, 50 :601-639
[5]   Runaway cell death, but not basal disease resistance, in Isd1 is SA- and NIM1/NPR1-dependent [J].
Aviv, DH ;
Rustérucci, C ;
Holt, BF ;
Dietrich, RA ;
Parker, JE ;
Dangl, JL .
PLANT JOURNAL, 2002, 29 (03) :381-391
[6]   Rapid, noninvasive screening for perturbations of metabolism and plant growth using chlorophyll fluorescence imaging [J].
Barbagallo, RP ;
Oxborough, K ;
Pallett, KE ;
Baker, NR .
PLANT PHYSIOLOGY, 2003, 132 (02) :485-493
[7]   Regulation and execution of programmed cell death in response to pathogens, stress and developmental cues [J].
Beers, EP ;
McDowell, JM .
CURRENT OPINION IN PLANT BIOLOGY, 2001, 4 (06) :561-567
[8]   Molecular identification and expression of the peroxidase responsible for the oxidative burst in French bean (Phaseolus vulgaris L.) and related members of the gene family [J].
Blee, KA ;
Jupe, SC ;
Richard, G ;
Zimmerlin, A ;
Davies, DR ;
Bolwell, GP .
PLANT MOLECULAR BIOLOGY, 2001, 47 (05) :607-620
[9]   The apoplastic oxidative burst in response to biotic stress in plants: a three-component system [J].
Bolwell, GP ;
Bindschedler, LV ;
Blee, KA ;
Butt, VS ;
Davies, DR ;
Gardner, SL ;
Gerrish, C ;
Minibayeva, F .
JOURNAL OF EXPERIMENTAL BOTANY, 2002, 53 (372) :1367-1376
[10]   DETECTION OF PICOMOLE LEVELS OF HYDROPEROXIDES USING A FLUORESCENT DICHLOROFLUORESCEIN ASSAY [J].
CATHCART, R ;
SCHWIERS, E ;
AMES, BN .
ANALYTICAL BIOCHEMISTRY, 1983, 134 (01) :111-116