A solid phase linker strategy for the direct synthesis of EDANS-labelled peptide substrates

被引：12

作者：

Beythien, J

White, PD

机构：

[1] Merck Biosci Ltd, Novabiochem, Nottingham NG9 2JR, England

[2] Merck Biosci AG, Novabiochem, CH-4448 Laufelfingen, Switzerland

来源：

TETRAHEDRON LETTERS | 2005年 / 46卷 / 01期

关键词：

FRET peptides; EDANS; Dabcyl; fluorescent enzyme substrate;

D O I：

10.1016/j.tetlet.2004.11.026

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

A novel linker strategy for the efficient synthesis of peptides C-terminally labelled with the EDANS fluorophore is described. Using this support. FRET peptide substrates bearing EDANS/Dabcyl fluorescent donor/acceptor groups can be readily prepared using standard Fmoc solid phase methods. (C) 2004 Elsevier Ltd. All rights reserved.

引用

页码：101 / 104

页数：4

共 10 条

[1]

DOERNER B, 1998, PEPTIDES 1998, P90

[2]

DRIJFHOUT JW, 1996, PEPTIDES CHEM STRUCT, P129

[3] NEW INTRAMOLECULARLY QUENCHED FLUOROGENIC PEPTIDE-SUBSTRATES FOR THE STUDY OF THE KINETIC SPECIFICITY OF PAPAIN [J].