Product monitoring and quantitation of oligosaccharides composition in agar hydrolysates by precolumn labeling HPLC

被引:32
作者
Chen, HM
Zheng, L
Lin, W
Yan, XJ
机构
[1] Ningbo Univ, Key Lab Marine Biotechnol, Ningbo 315211, Peoples R China
[2] Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
[3] Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
关键词
agarobiose oligomers; HPLC; alpha-naphthylamine; agaro-oligosaccharides;
D O I
10.1016/j.talanta.2004.04.002
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Since the discovery of multiple bioactivities for agarobiose oligomers, a quantitative method has been in great need to monitor the agarobiose oligomers. This report demonstrates that agarobiose oligomers can be separated with high resolution in HPLC after introducing a-naphthylamine into compounds. Agarobiose oligomers ranged from biose to decaose were isolated by Sephadex column. HPLC analysis indicated that each oliomer could be quantified with good linearity and a low detection limit of 0.1-4 mug/ml. The chromatographic profiles of agaro-oligosaccharides with different hydrolysis modes (hydrochloride, citric acid, solid acid, and hydroxyl radical degradation) showed that agarobiose could be obtained more than 57.8% using solid acid mediated hydrolysis, while hydrochloride acid could degrade agar into a series of agaro-oligosaccharides from biose to decaose. The yield of oligosaccharides was low if hydrolyzed by citric acid. The Fenton degradation can increase the speed of hydrolysis, but the product was complex. (C) 2004 Elsevier B.V. All rights reserved.
引用
收藏
页码:773 / 777
页数:5
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