Morphological and mitochondrial DNA divergence validates blackmouth, Galeus melastomus, and Atlantic sawtail catsharks, Galeus atlanticus, as separate species

被引:15
作者
Castilho, R.
Freitas, M.
Silva, G.
Fernandez-Carvalho, J.
Coelho, R.
机构
[1] Univ Algarve, BioCon, CCMAR, Biodivers & Conservat Grp, P-8005139 Faro, Portugal
[2] Univ Algarve, CCMAR, Coastal Fisheries Res Grp, P-8005139 Faro, Portugal
关键词
catsharks; delimiting species; elasmobranch; mtDNA; ND2; gene;
D O I
10.1111/j.1095-8649.2007.01455.x
中图分类号
S9 [水产、渔业];
学科分类号
0908 ;
摘要
A total of 60 morphometric traits and nucleotide sequences of the entire mtDNA NADH dehydrogenase subunit 2 (ND2) gene [1047 base pair (bp)] in 23 individuals of blackmouth, Galeus melastomus, and 13 individuals of sawtail catsharks, Galeus atlanticus, caught in Southern Portugal, were examined to test the validity of these two taxa. These sharks closely resemble each other, have overlapping geographical ranges and are difficult to identify by morphological characters. Non-metric multidimensional scaling of morphometric variables indicates a clear separation between the two species, with 10 characters each contributing 2.12-2.45% of the total variability between species. Maximum likelihood, parsimony and neighbour-joining trees revealed two major mtDNA haplotype clades, corresponding to the two species, with an average corrected sequence divergence between them of 3.39 +/- 0.56%. Within species divergences between haplotypes averaged 0.27 +/- 0.18% in G. melastomus and 0.12 +/- 0.08% in G. atlanticus. A total of 35 diagnostic nucleotide site differences and four restriction fragment length polymorphism recognition sites in the ND2 gene can be used to distinguish the two species. (c) 2007 The Authors Journal compilation (c) 2007 The Fisheries Society of the British Isles.
引用
收藏
页码:346 / 358
页数:13
相关论文
共 43 条
[11]  
DOUADY CJ, 2003, MOL PHYLOGENET EVOL, V26, P12
[12]  
DRUMMOND A, 2006, GENEIOUS
[13]   Global phylogeography of the scalloped hammerhead shark (Sphyrna lewini) [J].
Duncan, K. M. ;
Martin, A. P. ;
Bowen, B. W. ;
De Couet, H. G. .
MOLECULAR ECOLOGY, 2006, 15 (08) :2239-2251
[14]   A comparative study of the species composition of discards from five fisheries from the Algarve (southern Portugal) [J].
Erzini, K ;
Costa, ME ;
Bentes, L ;
Borges, TC .
FISHERIES MANAGEMENT AND ECOLOGY, 2002, 9 (01) :31-40
[15]  
FELSENSTEIN J, 1985, EVOLUTION, V39, P783, DOI 10.1111/j.1558-5646.1985.tb00420.x
[16]   Do genetically distinct rougheye rockfish sibling species differ phenotypically? [J].
Gharrett, A. J. ;
Mecklenburg, C. W. ;
Seeb, L. W. ;
Li, Z. ;
Matala, A. P. ;
Gray, A. K. ;
Heifetz, J. .
TRANSACTIONS OF THE AMERICAN FISHERIES SOCIETY, 2006, 135 (03) :792-800
[17]  
Greig TW, 2005, FISH B-NOAA, V103, P516
[18]  
GRIEKSPOOR A, 2006, ENZYME X
[19]   A simple, fast, and accurate algorithm to estimate large phylogenies by maximum likelihood [J].
Guindon, S ;
Gascuel, O .
SYSTEMATIC BIOLOGY, 2003, 52 (05) :696-704
[20]   DATING OF THE HUMAN APE SPLITTING BY A MOLECULAR CLOCK OF MITOCHONDRIAL-DNA [J].
HASEGAWA, M ;
KISHINO, H ;
YANO, TA .
JOURNAL OF MOLECULAR EVOLUTION, 1985, 22 (02) :160-174