共 112 条
Type IV secretion:: the Agrobacterium VirB/D4 and related conjugation systems
被引:159
作者:
Christie, PJ
[1
]
机构:
[1] Univ Texas, Dept Microbiol & Mol Genet, Sch Med, Houston, TX 77030 USA
来源:
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
|
2004年
/
1694卷
/
1-3期
关键词:
conjugation;
type IV secretion;
DNA transfer;
pathogenesis;
protein translocation;
pilus;
coupling protein;
D O I:
10.1016/j.bbamcr.2004.02.013
中图分类号:
Q5 [生物化学];
Q7 [分子生物学];
学科分类号:
071010 ;
081704 ;
摘要:
The translocation of DNA across biological membranes is an essential process for many living organisms. to bacteria, type W secretion systems (T4SS) are used to deliver DNA as well as protein substrates from donor to target cells. The T4SS are structurally complex machines. assembled from a dozen or more membrane proteins in response to environmental signals. In Gram-negative bacteria. the conjugation machines are composed of a cell envelope-spanning secretion channel and an extracellular pilus. These dynamic structures (i) direct formation of stable contacts-the mating junction-between donor and recipient cell membranes. (ii) transmit single-stranded DNA as a nucleoprotein particle, as well as protein substrates, across donor and recipient cell membranes, and (iii) mediate disassembly of die marine junction following substrate transfer. This review summarizes recent progress in our understanding of the mechanistic details of DNA trafficking with a focus on the paradigmatic Agrobacterium tumefaciens VirB/D4 T4SS and related conjugation systems. (C) 2004 Elsevier B.V. All rights reserved.
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页码:219 / 234
页数:16
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