Laboratory-scale production of lignin-degrading enzymes by free and entrapped cells of Phanerochaete chrysosporium in a tubular air-lift bioreactor

被引：12

作者：

Darah, I ^{[1
]}

Ibrahim, CO ^{[1
]}

机构：

[1] Univ Sains Malaysia, Sch Biol Sci, Fermentat & Enzyme Technol Lab, Minden 11800, Penang, Malaysia

来源：

FOLIA MICROBIOLOGICA | 1998年 / 43卷 / 02期

关键词：

D O I：

10.1007/BF02816503

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The production of lignin-degrading enzymes by free and entrapped cells of Phanerocoete chrysosporium in a tubular air-lift bioreactor was studied. Under optimized cultural conditions the production of lignin peroxidase by free cells, calcium-alginate-entrapped cells and scouring-mesh-entrapped cells was in a ratio of 520:720:950 mU/mL, while the production of manganese peroxidase was in a ratio of 350:480:620 mU/mL. The stability of the entrapped cells by fed-batch systems was highest after 3 feeding experiments which is similarly demonstrated in the repeated use of the preparations in batch system.

引用

页码：161 / 168

页数：8

共 35 条

[31] A NOVEL IMMOBILIZATION METHOD FOR PREVENTION OF CELL LEAKAGE FROM THE GEL MATRIX [J].