Synchronized infection of cell cultures by magnetically controlled virus

被引：38

作者：

Haim, H ^{[1
]}

Steiner, I

Panet, A

机构：

[1] Hebrew Univ Jerusalem, Fac Med, Dept Virol, Hadassah Med Sch, IL-91120 Jerusalem, Israel

[2] Hebrew Univ Jerusalem, Hadassah Med Sch, Lab Neurovirol, Dept Neurol, Jerusalem, Israel

来源：

JOURNAL OF VIROLOGY | 2005年 / 79卷 / 01期

关键词：

D O I：

10.1128/JVI.79.1.622-625.2005

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

To override the diffusion-limited adsorption step of viral infection, we magnetically synchronized cell attachment. Human immunodeficiency virus type 1-based lentivirus preparations were rendered magnetically reactive by association with magnetite nanoparticles, 50 nm in diameter. Application of a magnetic field resulted in immediate redistribution of the viral inoculum to the cell-associated state and completion of the productive adsorption process within 1 min. Independent of adsorption time, viral concentration, and diffusion rate, infection subsequently progressed by the receptor-mediated entry mechanism. Synchronization of this rate-limiting step of infection may now be applied to analyze isolated events in the viral replication sequence.

引用

页码：622 / 625

页数：4

共 29 条

[1] Viral mediated gene transfer to sprouting blood vessels during angiogenesis [J].

Alian, A ;

Eldor, A ;

Falk, H ;

Panet, A .

JOURNAL OF VIROLOGICAL METHODS, 2002, 105 (01) :1-11

[2] VIRUS PARTICLE ADSORPTION .3. ADSORPTION OF VIRUSES BY CELL MONOLAYERS AND EFFECTS OF SOME VARIABLES ON ADSORPTION [J].

ALLISON, AC ;

VALENTINE, RC .

BIOCHIMICA ET BIOPHYSICA ACTA, 1960, 40 (03) :400-410

[3] Toward a more accurate quantitation of the activity of recombinant retroviruses: Alternatives to titer and multiplicity of infection (vol 74, pg 1258, 2000) [J].

Andreadis, S ;

Lavery, T ;

Davis, HE ;

Le Doux, JM ;

Yarmush, ML ;

Morgan, JR .

JOURNAL OF VIROLOGY, 2000, 74 (07) :3431-3439

[4]

Axelrod B. N., 1999, J FORENSIC NEUROPSYC, V1, P15

[5] HIV-1 vif-derived peptide inhibits drug-resistant HIV proteases [J].

Blumenzweig, I ;

Baraz, L ;

Friedler, A ;

Danielson, UH ;

Gilon, C ;

Steinitz, M ;

Kotler, M .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2002, 292 (04) :832-840

[6] VESICULAR STOMATITIS-VIRUS G GLYCOPROTEIN PSEUDOTYPED RETROVIRAL VECTORS - CONCENTRATION TO VERY HIGH-TITER AND EFFICIENT GENE-TRANSFER INTO MAMMALIAN AND NONMAMMALIAN CELLS [J].

BURNS, JC ;

FRIEDMANN, T ;

DRIEVER, W ;

BURRASCANO, M ;

YEE, JK .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (17) :8033-8037

[7] Retroviral infection is limited by Brownian motion [J].

Chuck, AS ;

Clarke, MF ;

Palsson, BO .

HUMAN GENE THERAPY, 1996, 7 (13) :1527-1534

[8] Improved manufacture and application of an agarose magnetizable solid-phase support [J].

Davies, MJ ;

Smethurst, DE ;

Howard, KM ;

Todd, M ;

Higgins, LM ;

Bruce, IJ .

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 1997, 68 (1-2) :95-112

[9] Polybrene increases retrovirus gene transfer efficiency by enhancing receptor-independent virus adsorption on target cell membranes [J].

Davis, HE ;

Morgan, JR ;

Yarmush, ML .

BIOPHYSICAL CHEMISTRY, 2002, 97 (2-3) :159-172

[10] A third-generation lentivirus vector with a conditional packaging system [J].

Dull, T ;

Zufferey, R ;

Kelly, M ;

Mandel, RJ ;

Nguyen, M ;

Trono, D ;

Naldini, L .

JOURNAL OF VIROLOGY, 1998, 72 (11) :8463-8471

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