Synchronized infection of cell cultures by magnetically controlled virus

被引：38

作者：

Haim, H ^{[1
]}

Steiner, I

Panet, A

机构：

[1] Hebrew Univ Jerusalem, Fac Med, Dept Virol, Hadassah Med Sch, IL-91120 Jerusalem, Israel

[2] Hebrew Univ Jerusalem, Hadassah Med Sch, Lab Neurovirol, Dept Neurol, Jerusalem, Israel

来源：

JOURNAL OF VIROLOGY | 2005年 / 79卷 / 01期

关键词：

D O I：

10.1128/JVI.79.1.622-625.2005

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

To override the diffusion-limited adsorption step of viral infection, we magnetically synchronized cell attachment. Human immunodeficiency virus type 1-based lentivirus preparations were rendered magnetically reactive by association with magnetite nanoparticles, 50 nm in diameter. Application of a magnetic field resulted in immediate redistribution of the viral inoculum to the cell-associated state and completion of the productive adsorption process within 1 min. Independent of adsorption time, viral concentration, and diffusion rate, infection subsequently progressed by the receptor-mediated entry mechanism. Synchronization of this rate-limiting step of infection may now be applied to analyze isolated events in the viral replication sequence.

引用

页码：622 / 625

页数：4

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