Methodological Guidelines for Genetic Safety Testing of Cell Transplants

被引：6

作者：

Bochkov, N. P. ^{[1
,2
]}

Nikitina, V. A. ^{[1
]}

Voronina, E. S. ^{[1
]}

Kuleshov, N. P. ^{[2
]}

机构：

[1] Russian Acad Med Sci, Ctr Med Genet, Lab Mutagenesis, Moscow 109801, Russia

[2] IM Sechenov Moscow Med Acad, Moscow, Russia

来源：

BULLETIN OF EXPERIMENTAL BIOLOGY AND MEDICINE | 2009年 / 148卷 / 04期

基金：

俄罗斯基础研究基金会;

关键词：

methodological guidelines; cell transplant; multipotent mesenchymal stromal cells; karyotyping; aneuploidy; MESENCHYMAL STEM-CELLS; STROMAL CELLS; EXPANSION;

D O I：

10.1007/s10517-010-0793-7

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

100103 [病原生物学]; 100218 [急诊医学];

摘要：

A combination of karyotyping and aneuploidy analysis by interphase fluorescent in situ hybridization is a sensitive method for evaluation of genetic stability of stem cell cultures. The methodology and specific features of preparing and analyzing the cytogenetic preparations are described as exemplified by human multipotent mesenchymal stromal cells.

引用

页码：677 / 683

页数：7

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