Sperm chromatin structure assay parameters as predictors of failed pregnancy following assisted reproductive techniques

被引:351
作者
Larson, KL
DeJonge, CJ
Barnes, AM
Jost, LK
Evenson, DP
机构
[1] S Dakota State Univ, Dept Chem & Biochem, Brookings, SD 57007 USA
[2] Univ Minnesota, Dept Obstet & Gynecol, Minneapolis, MN 55454 USA
[3] Univ Nebraska, Med Ctr, Dept Obstet & Gynecol, Omaha, NE USA
关键词
chromatin structure; human fertility; in-vitro fertilization; intracytoplasmic sperm injection; SCSA;
D O I
10.1093/humrep/15.8.1717
中图分类号
R71 [妇产科学];
学科分类号
100211 ;
摘要
The predictive value of sperm chromatin integrity for pregnancy outcome following in-vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) was studied in 24 men attending a university-based assisted reproductive techniques laboratory using the flow cytometric sperm chromatin structure assay (SCSA). The SCSA is a measure of the susceptibility of sperm DNA to low pa-induced denaturation in situ. The mean percentage of spermatozoa in the neat sample demonstrating DNA denaturation was significantly lower in the seven men that initiated a pregnancy (15.4 +/- 4.6, P = 0.01) than in the 14 men who did not initiate a pregnancy (31.1 +/- 3.2). No pregnancies resulted if greater than or equal to 27% of the spermatozoa in the neat semen sample showed DNA denaturation. These data demonstrate that SCSA parameters are independent of conventional semen parameters. Furthermore, the SCSA may anew physicians to identify male patients for whom IVF and ICSI will be unlikely to result in pregnancy initiation.
引用
收藏
页码:1717 / 1722
页数:6
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