Decoding signalling networks by mass spectrometry-based proteomics

被引:437
作者
Choudhary, Chunaram [1 ]
Mann, Matthias [1 ,2 ]
机构
[1] Univ Copenhagen, Novo Nordisk Fdn, Ctr Prot Res, Fac Hlth Sci, DK-2200 Copenhagen, Denmark
[2] Max Planck Inst Biochem, Dept Prote & Signal Transduct, D-82152 Martinsried, Germany
关键词
CELL-CULTURE SILAC; QUANTITATIVE PHOSPHOPROTEOMICS; IN-VIVO; POSTTRANSLATIONAL MODIFICATIONS; PROTEIN COMPLEXES; GLOBAL ANALYSIS; HIGH-RESOLUTION; AMINO-ACIDS; PHOSPHORYLATION DYNAMICS; REVEALS;
D O I
10.1038/nrm2900
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Signalling networks regulate essentially all of the biology of cells and organisms in normal and disease states. Signalling is often studied using antibody-based techniques such as western blots. Large-scale 'precision proteomics' based on mass spectrometry now enables the system-wide characterization of signalling events at the levels of post-translational modifications, protein-protein interactions and changes in protein expression. This technology delivers accurate and unbiased information about the quantitative changes of thousands of proteins and their modifications in response to any perturbation. Current studies focus on phosphorylation, but acetylation, methylation, glycosylation and ubiquitylation are also becoming amenable to investigation. Large-scale proteomics-based signalling research will fundamentally change our understanding of signalling networks.
引用
收藏
页码:427 / 439
页数:13
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