Mutagenesis approaches to deduce structure-function relationships in terpene synthases

被引：75

作者：

Segura, MJR

Jackson, BE

Matsuda, SPT

机构：

[1] Rice Univ, Dept Chem, Houston, TX 77005 USA

[2] Rice Univ, Dept Biochem & Cell Biol, Houston, TX 77005 USA

来源：

NATURAL PRODUCT REPORTS | 2003年 / 20卷 / 03期

关键词：

SQUALENE-HOPENE CYCLASE; ENCODING CYCLOARTENOL SYNTHASE; SITE-DIRECTED MUTAGENESIS; ASPARTATE-RICH REGION; MOLECULAR-CLONING; OXIDOSQUALENE CYCLASE; ENZYMATIC CYCLIZATION; LANOSTEROL SYNTHASE; PENTALENENE SYNTHASE; TRICHODIENE SYNTHASE;

D O I：

10.1039/b008338k

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

This review highlights mutagenesis studies of terpene synthases, specifically sesquiterpene synthases and ovidosqualene cyclases. Mutagenesis studies of these enzymes have provided mechanistic insights, structure-function relationships for specific enzymatic residues, novel terpene structures and enzymes with novel activities The literature through, 2002 is reviewed and 113 references cited.

引用

页码：304 / 317

页数：14

共 114 条

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[112] SYNTHESIS OF (+)-5-EPI-ARISTOLOCHENE AND (+)-1-DEOXYCAPSIDIOL FROM CAPSIDIOL [J].

WHITEHEAD, IM ;

EWING, DF ;

THRELFALL, DR ;

CANE, DE ;

PRABHAKARAN, PC .

PHYTOCHEMISTRY, 1990, 29 (02) :479-482

[113] 5-EPI-ARISTOLOCHENE IS A COMMON PRECURSOR OF THE SESQUITERPENOID PHYTOALEXINS CAPSIDIOL AND DEBNEYOL [J].

WHITEHEAD, IM ;

THRELFALL, DR ;

EWING, DF .

PHYTOCHEMISTRY, 1989, 28 (03) :775-779

[114] Conversion of a plant oxidosqualene-cycloartenol synthase to an oxidosqualene-lanosterol cyclase by random mutagenesis [J].

Wu, TK ;

Griffin, JH .

BIOCHEMISTRY, 2002, 41 (26) :8238-8244

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