Identification of the biosynthetic units of Cypridina luciferin in Cypridina (Vargula) hilgendorfii by LC/ESI-TOF-MS

被引：25

作者：

Kato, S

Oba, Y

Ojika, M

Inouye, S

机构：

[1] Chisso Co, Yokohama Res Ctr, Kanazawa Ku, Yokohama, Kanagawa 2368605, Japan

[2] Nagoya Univ, Grad Sch Bioagr Sci, Chikusa Ku, Nagoya, Aichi 4648601, Japan

来源：

TETRAHEDRON | 2004年 / 60卷 / 50期

基金：

日本学术振兴会;

关键词：

bioluminescence; stable isotope; imidazopyrazinone; luciferase; biosynthesis;

D O I：

10.1016/j.tet.2004.09.080

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

In a luminous ostracod Cypridina (Vargula) hilgendorfii, Cypridina luciferin with an imidazopyrazinone structure (3,7-dihydroimidazopyrazin-3-one) is utilized for the luminescence reaction. To identify the biosynthetic units of Cypridina luciferin, the stable isotope labeled compounds were examined by feeding experiments with living Cypridina specimens. The incorporation of the labeled compounds into Cypridina luciferin was identified by the method of LC/ESI-TOF-MS analyses and these results suggested that L-tryptophan, L-arginine and L-isoleucine are structural units of Cypridina luciferin. (C) 2004 Elsevier Ltd. All rights reserved.

引用

页码：11427 / 11434

页数：8

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[11] IMAGING OF LUCIFERASE SECRETION FROM TRANSFORMED CHINESE-HAMSTER OVARY CELLS [J].