Enzymatic syntheses and selective hydrolysis of O-β-D-galactopyranosides using a marine mollusc β-galactosidase

被引：14

作者：

Giordano, A ^{[1
]}

Tramice, A ^{[1
]}

Andreotti, G ^{[1
]}

Mollo, E ^{[1
]}

Trincone, A ^{[1
]}

机构：

[1] CNR, Inst Chim Biomol, I-80072 Arco Felice Napoli, Italy

来源：

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS | 2005年 / 15卷 / 01期

关键词：

beta-galactosidase; Aplysia fasciata; enzymatic synthesis;

D O I：

10.1016/j.bmcl.2004.10.016

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

The use of crude extract of the hepatopancreas of Aplysia fasciata, a large mollusc belonging to the order Anaspidea containing a beta-galactosidase activity, was reported for the synthesis of different galactosides. Good yields with polar acceptors and the uncommon beta-1-3 selectivity in the transgalactosylation reactions with most of the acceptors were observed. A beta-1-2 selectivity in the hydrolytic conditions was also observed and discussed. (C) 2004 Elsevier Ltd. All rights reserved.

引用

页码：139 / 143

页数：5

共 19 条

[1] A direct enzymatic synthesis of beta-D-galactopyranosyl-D-xylopyranosides and their use to evaluate rat intestinal lactase activity in vivo [J].

Aragon, JJ ;

Canada, FJ ;

FernandezMayoralas, A ;

Lopez, R ;

MartinLomas, M ;

Villanueva, D .

CARBOHYDRATE RESEARCH, 1996, 290 (02) :209-216

[2]

CAREFOOT TH, 1987, OCEANOGR MAR BIOL, V25, P167

[3] Glycosidases and glycosyl transferases in glycoside and oligosaccharide synthesis [J].

Crout, DHG ;

Vic, G .

CURRENT OPINION IN CHEMICAL BIOLOGY, 1998, 2 (01) :98-111

[4]

EDWARDS M, 1988, J BIOL CHEM, V263, P4333

[5] Polysaccharolytic activity of the digestive enzymes of the macroalgal herbivore, Turbo sarmaticus (Mollusca: Vetigastropoda: Turbinidae) [J].

Foster, GG ;

Hodgson, AN ;

Boyd, CS .

COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY, 1999, 122 (01) :47-52

[6]

Fry SC, 1996, TRENDS PLANT SCI, V1, P326

[7] Chemoenzymatic synthesis of Gal(beta 1-3)Gal(beta 1-4)Xyl(beta)-L-Ser and Gal(beta 1-3)Gal(beta 1-4)Xyl(beta)-MU by the use of beta-D-galactosidase [J].

Fukase, K ;

Yasukochi, T ;

Suda, Y ;

Yoshida, M ;

Kusumoto, S .

TETRAHEDRON LETTERS, 1996, 37 (37) :6763-6766

[8] Galactosylation with beta-galactosidase from bovine testes employing modified acceptor substrates [J].

Gambert, U ;

Lio, RG ;

Farkas, E ;

Thiem, J ;

Bencomo, VV ;

Liptak, A .

BIOORGANIC & MEDICINAL CHEMISTRY, 1997, 5 (07) :1285-1291

[9] Enzymes in the synthesis of bioactive compounds:: the prodigious decades [J].

García-Junceda, E ;

García-García, JF ;

Bastida, A ;

Fernández-Mayoralas, A .

BIOORGANIC & MEDICINAL CHEMISTRY, 2004, 12 (08) :1817-1834

[10] Enzymatic synthesis of galactosyl-xylose by Aspergillus oryzae β-galactosidase [J].

Giacomini, C ;

Irazoqui, G ;

Gonzalez, P ;

Batista-Viera, F ;

Brena, BM .

JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC, 2002, 19 :159-165

← 1 2 →