Altered Lipid Droplet Dynamics in Hepatocytes Lacking Triacylglycerol Hydrolase Expression

被引:83
作者
Wang, Huajin [1 ,4 ]
Wei, Enhui [2 ,4 ]
Quiroga, Ariel D. [2 ,4 ]
Sun, Xuejin [5 ]
Touret, Nicolas [3 ]
Lehner, Richard [1 ,2 ,4 ]
机构
[1] Univ Alberta, Dept Cell Biol, Edmonton, AB T6G 2S2, Canada
[2] Univ Alberta, Dept Pediat, Edmonton, AB T6G 2S2, Canada
[3] Univ Alberta, Dept Biochem, Edmonton, AB T6G 2S2, Canada
[4] Univ Alberta, Grp Mol & Cell Biol Lipids, Edmonton, AB T6G 2S2, Canada
[5] Univ Alberta, Cross Canc Inst, Dept Oncol, Edmonton, AB T6G 1Z2, Canada
关键词
LOW-DENSITY LIPOPROTEIN; HORMONE-SENSITIVE LIPASE; PHOSPHOCHOLINE CYTIDYLYLTRANSFERASE-ALPHA; ADIPOSE TRIGLYCERIDE LIPASE; PLASMA HIGH-DENSITY; FATTY-ACID; ENDOPLASMIC-RETICULUM; APOLIPOPROTEIN-B; RAT HEPATOCYTES; HEPATIC CTP;
D O I
10.1091/mbc.E09-05-0364
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Lipid droplets (LDs) form from the endoplasmic reticulum (ER) and grow in size by obtaining triacylglycerols (TG). Triacylglycerol hydrolase (TGH), a lipase residing in the ER, is involved in the mobilization of TG stored in LDs for the secretion of very-low-density lipoproteins. In this study, we investigated TGH-mediated changes in cytosolic LD dynamics. We have found that TGH deficiency resulted in decreased size and increased number of LDs in hepatocytes. Using fluorescent fatty acid analogues to trace LD formation, we observed that TGH deficiency did not affect the formation of nascent LDs on the ER. However, the rate of lipid transfer into preformed LDs was significantly slower in the absence of TGH. Absence of TGH expression resulted in increased levels of membrane diacylglycerol and augmented phospholipid synthesis, which may be responsible for the delayed lipid transfer. Therefore, altered maturation (growth) rather than nascent formation (de novo synthesis) may be responsible for the observed morphological changes of LDs in TGH-deficient hepatocytes.
引用
收藏
页码:1991 / 2000
页数:10
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