New, stable halogenated derivative suitable for the gas chromatographic determination of muramic acid

被引：5

作者：

Biondi, PA ^{[1
]}

Chiesa, LM ^{[1
]}

Mariani, C ^{[1
]}

Renon, P ^{[1
]}

机构：

[1] IST ISPEZ ALIMENTI ORIGINE ANIM,I-20133 MILAN,ITALY

来源：

JOURNAL OF CHROMATOGRAPHY A | 1996年 / 726卷 / 1-2期

关键词：

derivatization; GC; muramic acid; peptidoglycan; glycans;

D O I：

10.1016/0021-9673(95)01234-6

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The formation of a new volatile derivative of muramic acid is described. The procedure entails a reaction with O-(pentafluorobenzyl)hydroxylamine in pyridine to give the corresponding oxime and a treatment with acetic anhydride in the presence of 4-N,N-dimethylaminopyridine. The structures of the obtained derivatives have been confirmed by MS. The influence of various catalyst amounts used during the acetylation step was studied and a mechanism was proposed in order to explain the different reaction courses.

引用

收藏

页码：246 / 252

页数：7

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