Antitumor effects of (1→3)-β-D-glucan and (1→6)-β-D-glucan purified from newly cultivated mushroom, Hatakeshimeji (Lyophyllum decastes Sing.)

被引:98
作者
Ukawa, Y
Ito, H
Hisamatsu, M [1 ]
机构
[1] Mie Univ, Fac Bioresources, Tsu, Mie 5148507, Japan
[2] Res Ctr Eishogen Co Ltd, Fukjaya, Saitama 3660815, Japan
[3] Mie Univ, Sch Med, Dept Pharmacol, Tsu, Mie 514, Japan
关键词
Hatakeshimeji (Lyophyllum decastes Sing.); antitumor activity; (1 -> 3)-beta-D-glucan; (1 -> 6)-beta-D-glucan; immunological host-mediated mechanism;
D O I
10.1016/S1389-1723(00)80041-9
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Eleven polysaccharides were isolated from a hot-water extract of fruiting bodies of Lyophyllum decastes Sing. by ion-exchange chromatography and gel permeation chromatography. Three polysaccharides (IV-I, IV-2, and IV-3) composed mainly of glucose showed marked antitumor activities against Sarcoma 180 and their average molecular weights were 305 kDa, 130 kDa and 14 kDa, respectively. From methylation analyses and C-13-NMR spectra, it was suggested that IV-I was a (1-->3)-beta-D-glucan-type polysaccharide, IV-3 was a (1-->6)-beta-D-glucan-type polysaccharide, and IV-2 was a (1-->3, 1-->6)-beta-D-glucan-type polysaccharide or a mixture of both polysaccharides. Increases in the number of peritoneal macrophages and third component of complement (C3)-positive fluorescent cells in mice treated with IV-1 suggested that the inhibitory effect on tumor growth is due to immunological host-mediated mechanisms.
引用
收藏
页码:98 / 104
页数:7
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